8.51025

5-Carboxyfluorescein

Novabiochem^®

• Synonyme(s) : 5-Carboxyfluorescein, 5-FAM
• Formule empirique (notation de Hill): C₂₁H₁₂O₇
• Numéro CAS: 76823-03-5
• Poids moléculaire : 376.32
• Numéro MDL: MFCD00036874
• Code UNSPSC : 12352005
• Nomenclature NACRES : NA.22

Propriétés

• Niveau de qualité: 200
• Gamme de produits: Novabiochem^®
• Pureté: ≥98% (TLC); ≥99.0% (HPLC)
• Forme: powder
• Pertinence de la réaction: reagent type: fluorescent-labelling reagent
• Fabricant/nom de marque: Novabiochem^®
• Application(s): peptide synthesis
• Température de stockage: 2-8°C
• InChI: 1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)
• Clé InChI: NJYVEMPWNAYQQN-UHFFFAOYSA-N

Description

Description générale

This high purity fluorescent dye (λ_ex494 nm, λ_em 518 nm) is supplied as a single isomer, which ensures production of labeled products of defined chemical structure, as well as greatly simplifying purification and characterization. FAM should be introduced as the last step in the synthesis to avoid formation of side-products, or be protected by tritylation prior to acylation or hydrazine treatment ^[1].

Associated Protocols and Technical Articles
Chromogenic Labeling of Synthetic Peptides

Literature references

^[1] R. Fischer, et al. (2003) Bioconjugate Chem., 14, 653.

Application

5-Carboxyfluorescein Novabiochem^®. CAS 76823-03-5, molar mass 376.32 g/mol.

Liaison

Replaces: 01-63-0112

Remarque sur l'analyse

Colour (visual): yellow to orange
Appearance of substance (visual): powder
Identity (IR): passes test
Purity (TLC (EtOAc/MeOH/H2O/DIPEA 60 : 30 : 10 : 3)): ≥ 98 %
Assay (HPLC, area%): ≥ 99.0 % (a/a)

To see the solvent systems used for TLC of Novabiochem^® products please click here.

Informations légales

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable