5.04744

Aphidicolin

≥98% (TLC), liquid, DNA polymerase α and δ inhibitor, Calbiochem

• Synonyme(s) : InSolution Aphidicolin
• Formule empirique (notation de Hill): C₂₀H₃₄O₄
• Numéro CAS: 38966-21-1
• Poids moléculaire : 338.48
• Numéro MDL: MFCD00083214
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.77

Propriétés

• product name: Aphidicolin, InSolution, ≥98%, 30 mM in DMSO, A cell-permeable tetracyclic diterpene antibiotic
• Niveau de qualité: 100
• Pureté: ≥98% (TLC)
• Forme: liquid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles; desiccated (hygroscopic); protect from light
• Température de stockage: −70°C
• InChI: 1S/C20H34O4/c1-17(11-21)15-4-3-13-9-14-10-19(13,7-8-20(14,24)12-22)18(15,2)6-5-16(17)23/h13-16,21-24H,3-12H2,1-2H3/t13-,14+,15-,16+,17-,18-,19-,20-/m0/s1
• Clé InChI: NOFOAYPPHIUXJR-APNQCZIXSA-N

Description

Description générale

A cell-permeable tetracyclic diterpene antibiotic. Cell synchronization agent. Blocks the cell cycle at the early S-phase. Specific, non-competitive and reversible inhibitor of DNA polymerase α and δ in eukaryotic cells and in some viruses of animal origin. Potentiates apoptosis induced by arabinosyl nucleosides in leukemia cell lines. Also induces apoptosis in HeLaS3 cells, but inhibits vincristine (>Cat. No. 677181)-induced apoptosis in the p53-negative human prostate cancer cell line PC-3.

Actions biochimiques/physiologiques

Cell permeable: yes

Primary Target
DNA polymerase α and δ

Reversible: yes

Conditionnement

Packaged under inert gas

Avertissement

Toxicity: Irritant (B)

Forme physique

A 30 mM (1 mg/98 µL) sterile-filtered solution of Aphidicolin (Cat. No. 178273) in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 6 months at -70°C. Use only fresh DMSO for further dilutions.

Autres remarques

Borner, M.M., et al. 1995. Cancer Res.55, 2122.
Poluha, W., et al. 1995. Oncogene10, 185.
Urbani, L., et al. 1995. Exp. Cell Res.219, 159.Schimke, R.T., et al. 1994. Philos. Trans. R. Soc. London. B. Biol. Sci.345, 311.Kuwakado, K., et al. 1993. Biochem. Pharmacol.46, 1909.Hubermann, J.A. 1981. Cell23, 647.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): 188.6 °F - (refers to pure substance)
• Point d'éclair (°C): 87 °C - (refers to pure substance)