345806-M

β1,4-Galactosidase, Streptococcus pneumoniae, Recombinant, E. coli

• Synonyme(s) : β1,4-Galactosidase, Streptococcus pneumoniae, Recombinant, E. coli, β1,4-D-Galactoside Galactohydrolase
• Numéro CAS: 9031-11-2
• Numéro de classification (Commission des enzymes): 3.2.1.23 (BRENDA, IUBMB)
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Produit recombinant: expressed in E. coli
• Niveau de qualité: 200
• Forme: liquid
• Activité spécifique: ≥1.5 units/mL; ≥6 units/mg protein
• Conditionnement: pkg of 50 mIU
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: do not freeze
• Activité étrangère: N-acetylglucosaminidase, α-galactosidase, α-mannosidase, neuraminidases, proteases, none detected
• Température de stockage: 2-8°C

Description

Description générale

Note: 1 mU = 1 milliunit.

Recombinant, Streptococcus pneumoniae β1,4-Galactosidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal β1,4-linked galactose. This enzyme is not known to cleave substituted or branched galactose. Inhibited by fucose attached to penultimate sugar.

Application

Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-β-D-galactopyranoside per min at 37°C, pH 5.0.

Forme physique

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5. Sterile solution.

Autres remarques

Kierman, U.A., et al. 2004. Proteomincs4, 1825.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable