1.04178

Rhodamine B (C.I.45170)

• Formule empirique (notation de Hill): C₂₈H₃₁ClN₂O₃
• Numéro CAS: 81-88-9
• Poids moléculaire : 479.01
• Numéro MDL: MFCD00011931
• Code UNSPSC : 12352116
• Nomenclature NACRES : NA.21

Propriétés

• Forme: solid
• Niveau de qualité: 200
• Puissance: >2000 mg/kg LD₅₀, oral (Rat)
• Composition: Dye, ≥90% spectrophotometric assay
• pH: 3-4 (20 °C, 10 g/L in H₂O)
• Pf: 199-201 °C
• Solubilité: 15 g/L
• Densité: 1.31 g/cm³ at 20 °C
• Masse volumique apparente: 250 kg/m³
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: 2-30°C
• InChI: 1S/C28H30N2O3.ClH/c1-5-29(6-2)19-13-15-23-25(17-19)33-26-18-20(30(7-3)8-4)14-16-24(26)27(23)21-11-9-10-12-22(21)28(31)32;/h9-18H,5-8H2,1-4H3;1H
• Clé InChI: PYWVYCXTNDRMGF-UHFFFAOYSA-N

Description

Description générale

Rhodamine B (C.I. 45170) for microscopy, is a solid dye for fluorescence AFB staining of bacteriological smears and histological tissue sections of human origin. The mechanism is a non-specific binding of fluorescence dye on a substrate, similar to the classical Ziehl-Neelsen method, although the heating of the Rhodamine B color solution can be avoided. The dye has a constant dye content and a reliable high quality, which is one of the major precondition for a reproducible diagnostic staining.

Application

for microscopy

Remarque sur l'analyse

Dye content (spectrophotometrically): ≥ 90 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (ethanol 50 %): 550 - 552 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.003 g/l; ethanol 50 %): 2115 - 2350
TLC-Test: passes test
Loss on drying (110 °C): ≤ 5 %
Suitability for microscopy: passes test

Informations sur la sécurité

• Pictogrammes: GHS05,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H302 - H318 - H412
• Conseils de prudence: P264 - P270 - P273 - P280 - P301 + P312 - P305 + P351 + P338
• Classification des risques: Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable