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R1755

Sigma-Aldrich

Rhodamine B isothiocyanate

mixed isomers, BioReagent, suitable for protein labeling

Synonyme(s) :

RBITC

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About This Item

Formule empirique (notation de Hill):
C29H30ClN3O3S
Numéro CAS:
36877-69-7
Poids moléculaire :
536.08
Numéro CE :
253-248-9
Numéro MDL:
MFCD00136007
Code UNSPSC :
12352116
ID de substance PubChem :
329824007
Nomenclature NACRES :
NA.32

Description

labeling efficiency with bovine albumin > 70%

Niveau de qualité

200

Gamme de produits

BioReagent

Fluorescence

λex 543 nm; λem 580 nm in methanol

Adéquation

suitable for protein labeling

Température de stockage

−20°C

Chaîne SMILES 

[Cl-].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4C(O)=O)N=C=S)=[N+](\CC)CC)c1

InChI

1S/C29H29N3O3S.ClH/c1-5-31(6-2)20-10-13-23-26(16-20)35-27-17-21(32(7-3)8-4)11-14-24(27)28(23)22-12-9-19(30-18-36)15-25(22)29(33)34;/h9-17H,5-8H2,1-4H3;1H

Clé InChI

YVSWPCCVTYEEHG-UHFFFAOYSA-N

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Catégories apparentées

Description générale

Rhodamine B isothiocyanate (RhBITC) is an organic fluorescence dye and is widely used as a fluorescent tag in a variety of biological applications. RhBITC is adsorbed onto Ag on silica or polystyrene beads. It exhibits not only a strong surface-enhanced Raman scattering (SERS) signal but also a measurable amount of fluorescence. The RhBITC-modified Ag-deposited silica or polystyrene beads disperse well in ethanol. They are also readily coated in water with polyelectrolytes for their further derivatization with biological molecules of interest that can bind to target molecules.

Application

Rhodamine B isothiocyanate is used for the following applications:
  • Rhodamine isothiocyanate labeling
  • Rhodamine-labeled synthetic APF
  • Preparations of protein and fluorescent conjugates
  • Fluorescent red dye

Pictogrammes

Exclamation markHealth hazard
GHS07,GHS08

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Jun-Sung Kim et al.
Journal of veterinary science, 7(4), 321-326 (2006-11-16)
Biocompatible silica-overcoated magnetic nanoparticles containing an organic fluorescence dye, rhodamine B isothiocyanate (RITC), within a silica shell [50 nm size, MNP@SiO2(RITC)s] were synthesized. For future application of the MNP@SiO2(RITC)s into diverse areas of research such as drug or gene delivery
S K Williams et al.
Proceedings of the National Academy of Sciences of the United States of America, 78(4), 2393-2397 (1981-04-01)
Microvessels isolated from rat epididymal fat exhibit differential vesicular ingestion rates for unmodified and non-enzymatically glycosylated rat albumin. While unmodified rat albumin is excluded from ingestion by endothelial micropinocytic vesicles, glycosylated albumin is avidly taken up by endocytosis. Interaction of
Control Over Aragonite Crystal Nucleation and Growth: An In Vitro Study of Biomineralization.
Yael Levi et al.
Chemistry (Weinheim An Der Bergstrasse, Germany), 4 (1998)
Kwan Kim et al.
Biosensors & bioelectronics, 24(7), 1864-1869 (2008-11-05)
Rhodamine B isothiocyanate (RhBITC) is a prototype dye molecule that is widely used as a fluorescent tag in a variety of biological applications. We report in this work that once RhBITC is adsorbed onto Ag on silica or polystyrene beads
Mengying Zhang et al.
Lab on a chip, 10(9), 1199-1203 (2010-04-15)
We report a simple methodology to fabricate PDMS multi-layer microfluidic chips. A PDMS slab was surface-treated by trichloro (1H,1H,2H,2H-perfluorooctyl) silane, and acts as a reusable transferring layer. Uniformity of the thickness of the patterned PDMS layer and the well-alignment could
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