05-583
Anti-CFTR Antibody, clone M3A7
clone M3A7, Upstate®, from mouse
Synonyme(s) :
ATP-binding cassette sub-family C, member 7, ATP-binding cassette transporter sub-family C member 7, cAMP-dependent chloride channel, cystic fibrosis transmembrane conductance regulator, cystic fibrosis transmembrane conductance regulator (ATP-binding ca
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
M3A7, monoclonal
Espèces réactives
human
Fabricant/nom de marque
Upstate®
Technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotype
IgG1
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... CFTR(1080)
Description générale
CFTR (Cystic Fibrosis Transmembrane Regulator) is an integral membrane protein shown to be involved in the transport of chloride ions. It is found on the surface of the epithelial cells lining the lungs and other organs. CFTR defects are the cause of cystic fibrosis. Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations of the gene coding for the cystic fibrosis transmembrane conductance factor, a cAMP regulated chloride channel. Approximately 70% of all CF cases share the deletion of a phenylalanine at position 508 (delta F508), which results in abnormal chloride transport. Since the CF mutation is lethal, most often by lung and liver disease, it raises the question of why this genetic disease remains as common as it is.
Spécificité
Other species not tested.
This antibody recognizes CFTR, Mr 170 kDa.
Immunogène
Epitope: a.a. 1365-1395
GST-fusion protein corresponding to residues 1197-1480 of human cystic fibrosis transmembrane conductance regulator (CFTR). The epitope maps within amino acids 1365-1395. Clone M3A7.
Application
Detect CFTR using this Anti-CFTR Antibody, clone M3A7 validated for use in IH, IP & WB.
Immunoprecipitation:
This antibody has been reported to immunoprecipitate CFTR. (Kartner, N., 1998.)
Immunohistochemistry:
This antibody has been reported to immunostain CFTR in human pancreatic tissue sections. (Kartner, N., 1998.)
This antibody has been reported to immunoprecipitate CFTR. (Kartner, N., 1998.)
Immunohistochemistry:
This antibody has been reported to immunostain CFTR in human pancreatic tissue sections. (Kartner, N., 1998.)
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Qualité
Routinely evaluated by western blot on human T84 colon carcinoma epithelial RIPA cell lysates or from CFTR-transfected BHK2.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected CFTR from 20-50 µg of human T84 colon carcinoma epithelial RIPA cell lysates. 0.5-2 µg/mL of a previous lot detected CFTR from CFTR-transfected BHK (Haardt, M., 1999).
Note: Do not boil the lysate. Instead incubate at 37°C for 30 minutes. CFTR can run as a diffuse protein on SDS-PAGE.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected CFTR from 20-50 µg of human T84 colon carcinoma epithelial RIPA cell lysates. 0.5-2 µg/mL of a previous lot detected CFTR from CFTR-transfected BHK (Haardt, M., 1999).
Note: Do not boil the lysate. Instead incubate at 37°C for 30 minutes. CFTR can run as a diffuse protein on SDS-PAGE.
Description de la cible
170 kDa
Forme physique
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol.
Stockage et stabilité
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Remarque sur l'analyse
Control
T84 cell lysate.
T84 cell lysate.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Mark R Silvis et al.
The Journal of biological chemistry, 278(13), 11554-11560 (2003-01-17)
Cystic fibrosis is a common lethal genetic disease among Caucasians. The cystic fibrosis gene encodes a cyclic adenosine monophosphate-activated chloride channel (cystic fibrosis transmembrane conductance regulator (CFTR)) that mediates electrolyte transport across the luminal surfaces of a variety of epithelial
M Haardt et al.
The Journal of biological chemistry, 274(31), 21873-21877 (1999-07-27)
Defective cAMP-stimulated chloride conductance of the plasma membrane of epithelial cell is the hallmark of cystic fibrosis (CF) and results from mutations in the cystic fibrosis transmembrane conductance regulator, CFTR. In the majority of CF patients, mutations in the CFTR
Marybeth Howard et al.
The Journal of biological chemistry, 278(37), 35159-35167 (2003-07-03)
In cystic fibrosis, the absence of functional CFTR results in thick mucous secretions in the lung and intestines, as well as pancreatic deficiency. Although expressed at high levels in the kidney, mutations in CFTR result in little or no apparent
Nicoletta Pedemonte et al.
American journal of physiology. Cell physiology, 298(4), C866-C874 (2010-01-08)
Cystic fibrosis (CF) is caused by mutations in the CFTR chloride channel. Deletion of phenylalanine 508 (F508del), the most frequent CF mutation, impairs the maturation and gating of the CFTR protein. Such defects may be corrected in vitro by pharmacological
Miquéias Lopes-Pacheco et al.
Chembiochem : a European journal of chemical biology, 17(6), 493-505 (2016-02-13)
We evaluated whether small molecule correctors could rescue four nucleotide-binding domain 1 (NBD1) mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene (A455E, S492F, ΔI507, and R560T). We first transfected Cos-7 cells (green monkey kidney cells) with A455E, S492F, ΔI507
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