04-745

Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315, rabbit monoclonal

culture supernatant, clone MC315, Upstate^®

• Synonyme(s) : H3K4me3, Histone H3 (tri methyl K4)
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: culture supernatant
• Type de produit anticorps: primary antibodies
• Clone: MC315, monoclonal
• Espèces réactives: human
• Fabricant/nom de marque: Upstate^®
• Technique(s): ChIP: suitable (ChIP-seq); dot blot: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); multiplexing: suitable; western blot: suitable
• Isotype: IgG
• Numéro d'accès NCBI: NM_003493
• Numéro d'accès UniProt: Q16695
• Conditions d'expédition: dry ice
• Modification post-traductionnelle de la cible: trimethylation (Lys4)
• Informations sur le gène: human ... H3C1(8350)

Description

Description générale

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.



The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Spécificité

Histone H3 containing trimethyl-lysine 4 and, to a lesser extent, dimethyl-lysine 4. See COA for specificity data.

Immunogène

Peptide containing the sequence [RT_trimKQ] in which lysine 4 is trimethylated on human histone H3

Application

Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315 is a rabbit monoclonal antibody for detection of trimethyl-Histone H3 (Lys4) also known as H3K4me3, Histone H3 (tri methyl K4) & has been validated in WB, ChIP, DB, Mplex, ChIP-seq.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected trimethyl-Histone H3 (Lys4) in human colon and human breast cancer tissue sections

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected trimethyl-Histone H3 (Lys4) in HeLa, A431, HUVEC, and NIH3T3 cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Qualité

routinely evaluated by immunoblot on in acid extracted proteins from HeLa cells, but not recombinant unmethylated Histone H3 (Catalog #14-494)

Description de la cible

17 kDa

Liaison

Replaces: 04-791

Forme physique

Cultured supernantant in 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Remarque sur l'analyse

Control
HeLa acid extract

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2