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53569-U

Supelco

Ascentis® Express 90 Å F5 (2.7 μm) HPLC Columns

L × I.D. 10 cm × 2.1 mm, HPLC Column

Synonym(s):

Core-shell (SPP) Fused Core PFP HPLC column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Ascentis® Express F5, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

material

stainless steel column

Agency

suitable for ISO 21675 2019
suitable for USP L43

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

parameter

60 °C temp. range
600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

10 cm × 2.1 mm

surface area

135 m2/g

impurities

<5 ppm metals

matrix

Fused-Core particle platform
superficially porous particle

matrix active group

PFP (pentafluorophenyl) phase

particle size

2.7 μm

pore size

90 Å pore size

operating pH

2-8

application(s)

food and beverages

separation technique

reversed phase

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General description

The pentafluorophenylpropyl stationary phase of Ascentis Express F5 provides a stable reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines.In addition to forming pi-pi and mildly steric interactions, F5 phases also retain compounds by polar interactions. Ascentis Express F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Application



  • Interference of Preservatives on Urinary Iodine Measurement by Sandell-Kolthoff Method.: This research examines the impact of preservatives, including hydrochloric acid, on the accuracy of urinary iodine measurements. The study highlights the need for careful selection of preservatives to avoid analytical interference (Kaya et al., 2022).


  • Monitoring the Extraction of Copper from Chicken Dung Leachate Using an Aluminium Electrode as an Indicator.: The study explores the use of hydrochloric acid in the leaching process of copper from chicken dung. It demonstrates the effectiveness of hydrochloric acid in extracting metals from organic waste materials (Kugeria et al., 2019).


  • Development of an Analytical Method for Urocanic Acid Isomers in Fish Based on Reactive Extraction Cleanup and Chaotropic Chromatography Techniques.: This paper details a method using hydrochloric acid for the extraction and analysis of urocanic acid isomers in fish. The method enhances the detection and quantification of these compounds in biological samples (Zhong et al., 2018).


  • Determination of Single-Ion Activities of H+ and Cl- in Aqueous Hydrochloric Acid Solutions by Use of an Ionic Liquid Salt Bridge.: The research provides a novel approach to determining the activities of hydrogen and chloride ions in hydrochloric acid solutions using an ionic liquid salt bridge, contributing to the understanding of ion behavior in aqueous solutions (Sakaida et al., 2011).




Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Application

Product No.
Description
Pricing

required but not provided

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Iltaf Shah et al.
The Journal of steroid biochemistry and molecular biology, 180, 118-128 (2018-02-13)
Research shows that immunoassay techniques are not the best choice for the estimation of vitamin D in human blood samples. The main reasons are that some immunoassays are not able to distinguish between 25-OHD3 and 25-OHD2 vitamin D metabolites. Furthermore

Articles

A significantly improved HPLC-fluorescence method for DMB-NANA and -NGNA, and application of this method to compare 2 candidate biosimilar therapeutic proteins to their respective RMs.

This Sigma-Aldrich article continues to detail new methodology for the analysis of Vitamin D metabolites using HybridSPE-Phospholipid technology.

Improvement in LC/MS Analysis of Vitamin D Metabolites in Serum by Leveraging Column Selectivity and Effective Sample Prep

Enhanced selectivity for stereoisomers and closely related compounds with alternate selectivity from C18 phases for basic, acidic, or neutral compounds

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Protocols

Learn more about LC/MS/MS Analysis using on-line cartridges for the removal of phospholipids from protein precipitated biological fluid samples

LC/MS Analysis of Opioid Glucuronide Metabolites in Urine on Ascentis® Express F5 after Solid Phase Extraction (SPE) using Supel™-Select HLB

Fast and Accurate LC-MS Analysis of Vitamin D Metabolites, Using Ascentis® Express F5 HPLC Columns from Supelco®

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