53829-U
Ascentis® Express 90 Å C18 (2.7 μm) HPLC Columns
L × I.D. 15 cm × 4.6 mm, HPLC Column
Synonym(s):
Core-shell (SPP) Fused Core C18 HPLC column
About This Item
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product name
Ascentis® Express C18, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 15 cm × 4.6 mm
material
stainless steel column
Quality Level
Agency
suitable for USP L1
product line
Ascentis®
feature
endcapped
manufacturer/tradename
Ascentis®
packaging
1 ea of
parameter
60 °C temp. range
600 bar max. pressure (9000 psi)
technique(s)
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
L × I.D.
15 cm × 4.6 mm
surface area
135 m2/g
impurities
<5 ppm metals
matrix
Fused-Core particle platform
superficially porous particle
matrix active group
C18 (octadecyl) phase
particle size
2.7 μm
pore size
90 Å
operating pH
2-9
application(s)
food and beverages
separation technique
reversed phase
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General description
Visit the Ascentis Express home page for more information on this new column technology.
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Articles
Compendial methods from the USP (United States Pharmacopeia) are widely used in pharmaceutical drug product and raw materials testing. However, not all methods in the USP use modern technologies. In chromatographic methods, it is not uncommon that older brands of columns are specified. Therefore, the USP methods are under continuous revision to improve existing procedures or to allow the user to obtain better results.
Apixaban is sold under the tradename Eliquis and at present no pharmacopeial monographs are available for Axpixaban using either an assay and/or organic impurity profiling method. A newly developed and validated HPLC-UV method using a column with C18 solid core particles is demonstrated.
Derivatization of Aliphatic Amines - Results from the Supelco Ascentis Express column show higher peak resolution, and improved peak shapes than typical chromatograms.
Sigma-Aldrich presents an article on serum lipidomics profiling using an Ascentis Express Fused-Core C18 column.
Protocols
This paper illustrates the setting up of a Lopinavir assay following European pharmacopoeia guidelines (10.2), and using a shorter column packed with smaller particles. The described assay saves valuable time and improves separation efficiency, which typically translates to better method performance.
This method is particularly useful in research into the role of individual bile acids as signaling molecules; suitable for clinical laboratories to investigate potential mechanisms linked to gut hormone profiles and glycemic control.
HPLC Analysis of Benzene and Deuterated Benzene on Ascentis® Express C18
Related Content
Ginsenosides Separation in Ginseng. The HPLC method was first optimized using a ginsenoside standard mixture, and was then applied to a sample of American Ginseng root.
The application note describes the separation of Ibuprofen and its related impurities using an Ascentis® Express 90Å C18 column with excellent peak shape and limits of quantification of 1 ppm or less.
Bile Acids (BA) are synthesized in the liver and play important roles in cholesterol homeostasis, absorption of vitamins and lipids, and various key metabolic processes.
Chromatograms
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