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45250

Sigma-Aldrich

Eosin Methylene blue

for microscopy (Bact.)

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About This Item

Colour Index Number:
45380/52015
MDL number:
MFCD00005038
UNSPSC Code:
12352200
PubChem Substance ID:
329756624
NACRES:
NA.47

grade

for microscopy (Bact.)

form

solid

SMILES string

[K+].COC(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24

InChI

1S/C21H10Br4O5.K/c1-29-21(28)9-5-3-2-4-8(9)14-10-6-12(22)17(26)15(24)19(10)30-20-11(14)7-13(23)18(27)16(20)25;/h2-7,26H,1H3;/q;+1/p-1

InChI key

NXBZXYJXNIFCRJ-UHFFFAOYSA-M

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General description

Mixture of eosin and methylene blue stains for use in differential microbiological media to culture and identify pathogenic microorganisms, e.g., Neisseria sicca,, Acinetobacter calcoaceticus.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302 - H317 - H319

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Eosin B certified by the Biological Stain Commission, Dye content 90 %

Sigma-Aldrich

861006

Eosin B

D F Spino et al.
Applied and environmental microbiology, 41(4), 1063-1064 (1981-04-01)
Acinetobacter calcoaceticus, frequently found in drinking waters and implicated in nosocomial infections, was presumptively identified by its tiny, blue colonial appearance on Levine eosin methylene blue agar. All of the 33 isolates from drinking water showing this distinctive colonial appearance

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