HPLC Analysis of Xylose, Fructose, Glucose and Saccharose on Chromolith^® NH2 100 - 4.6mm

column

Chromolith^® NH2 100 x 4.6mm (1.52028.0001)

mobile phase

[A] acetonitrile; [B] water; (75:25, A:B)

flow rate

1.0 mL/min

pressure

160 psi (11 bar)

column temp.

23 °C ambient

detector

190 nm cell: 11 μL

injection

2 μL

sample

Xylose 2.28 mg/mL, Fructose 6.45 mg/mL, Glucose 9.20 mg /mL, and Saccharose 9.42 mg/mL diluted in mobile phase.