OSHA Method 42/47 and ASTM® D5836: HPLC Analysis of Isocyanates on SUPELCOSIL™ LC-8 after Collection/Desorption using ORBO™-80
CONDITIONS
sample/matrix
calibration standard: isocyanate derivatives, 5 μg/mL in acetonitrile blank: 1-(2-pyridyl)piperazine (1-2PP) coated glass fiber filter desorbed in acetonitrile/DMSO, 9:1
adsorbent tube
ORBO-80 (20811)
column
SUPELCOSIL LC-8, 25 cm × 4.6 mm I.D., 5 μm particles (58297)
mobile phase
0.05 M ammonium acetate in water:acetonitrile, 70:30 (pH 6-6.2 with acetic acid)
flow rate
linear gradient, 0.7 mL/min to 2 mL/min in 15 min
detector
UV, 254 nm
injection
10 μL
Beschreibung
Rechtliche Hinweise
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ORBO is a trademark of Sigma-Aldrich Co. LLC
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC