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LC/MS/MS Analysis of Carbendazim in Orange Juice (Spiked) on Ascentis® Express C18 after Supel QuE PSA/C18 QuEChERS Cleanup

LC/MS/MS Analysis of Carbendazim in Orange Juice (Spiked) on Ascentis® Express C18 after Supel™ QuE PSA/C18 QuEChERS Cleanup application for HPLC, application for SPE

Materialien

Analytische Säule

Produkt-Nr.
Beschreibung
Preisangaben

Ascentis® Express C18, 2,7 μm HPLC-Säule

2.7 μm particle size, L × I.D. 5 cm × 2.1 mm

SPE-Röhrchen oder -Platte

Produkt-Nr.
Beschreibung
Preisangaben

Supel QuE

Citrate Extraction Tube, pk of 50, suitable for EN 15662

Standard

Produkt-Nr.
Beschreibung
Preisangaben

Carbendazim

PESTANAL®, analytical standard

Ähnliches Produkt

Produkt-Nr.
Beschreibung
Preisangaben

Supel QuE

Empty Centrifuge Tube with Lid, centrifuge tube volume 50 mL, pk of 50, suitable for EN 15662:2008 per BS

Supel QuE

PSA Tube, pack of 100, AOAC® 2007.01

Supel QuE

PSA/C18 Tube, pack of 100, AOAC® 2007.01

Supel QuE

Non-Buffered Tube 1, pk of 50

CONDITIONS

sample preparation

Dispersive (QuEChERS)

sample/matrix

orange juice spiked with carbendazim at 1 ppb

extraction tube

Supel QuE Citrate (EN) Tube (55227-U)

extraction process

add 10 mL of orange juice with pulp to a 50 mL empty extraction tube (55248-U); add carbendazim at 1 ppb; add 10 mL acetonitrile; (shake for 1 minute; add contents of citrate extraction tube (55227-U); shake for 1 min; centrifuge at 3200 rpm for 5 minutes)

clean-up tube

Supel-Q PSA/C18 (55288-U)

clean-up process

transfer 0.7 mL of the acetonitrile layer to the PSA/C18 cleanup tube (55288-U); shake 1 minute; (centrifuge for 3 minutes at 3400 rpm; remove 0.25 mL of the supernatant; mix with 0.25 mL water for LC-MS analysis)

column

Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 μm particles (53822-U)

mobile phase

[A] 10 mM ammonium acetate in water; [B] 10 mM ammonium acetate in methanol

gradient

0-1 min: 30% B; 1.5-3.5 min: 100% B; 3.5-7 min: 30% B

flow rate

0-1 min: 0.3 mL/min; 1.5-7 min: 0.5 mL/min

column temp.

30 °C

detector

MS, ESI(+), MRM, m/z 192/160, 192/132

injection

5 μL

Beschreibung

Hinweis zur Analyse

Simple and fast extraction and analytical method were developed for analysis of fungicide carbendazim in orange juice samples. QuEChERS extraction method was used with PSA/C18 cleanup. Ascentis Express C18 column chromatography was coupled to MS/MS detection with limits of detection at 0,1 ppb.

Rechtliche Hinweise

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Supel is a trademark of Sigma-Aldrich Co. LLC
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