LC/MS Analysis of Methadone and Metabolites EDDP and EMDP on Ascentis® Express RP Amide
Materialien
Analytische Säule
Produkt-Nr.
Beschreibung
Preisangaben
Standard
Produkt-Nr.
Beschreibung
Preisangaben
(±)-Methadon -Lösung
1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®EDDP perchlorate solution
1.0 mg/mL in methanol (as pyrolinium), ampule of 1 mL, certified reference material, Cerilliant®EMDP hydrochloride solution
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express RP Amide, 10 cm x 2.1 mm I.D., 2.7 μm particles (53913-U)
mobile phase
[A] 10 mM ammonium formate in water, pH 3.6; [B] 10 mM ammonium formate in acetonitrile, pH 3.6; (65:35, A:B)
flow rate
0.5 mL/min
column temp.
35 °C
detector
ESI(+), m/z 100-1000
injection
0.5 μL
sample
200 μg/L in 25:75, water: acetonitrile
Beschreibung
Rechtliche Hinweise
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany