Small interfering RNA delivery by polyethylenimine-functionalised porous silicon nanoparticles.

Small interfering RNA delivery by polyethylenimine-functionalised porous silicon nanoparticles.

Biomaterials science (2015-09-08)

M Hasanzadeh Kafshgari, M Alnakhli, B Delalat, S Apostolou, F J Harding, E Mäkilä, J J Salonen, B J Kuss, N H Voelcker

ZUSAMMENFASSUNG

In this study, thermally hydrocarbonised porous silicon nanoparticles (THCpSiNPs) capped with polyethylenimine (PEI) were fabricated, and their potential for small interfering RNA (siRNA) delivery was investigated in an in vitro glioblastoma model. PEI coating following siRNA loading enhanced the sustained release of siRNA, and suppressed burst release effects. The positively-charged surface improved the internalisation of the nanoparticles across the cell membrane. THCpSiNP-mediated siRNA delivery reduced mRNA expression of the MRP1 gene, linked to the resistence of glioblastoma to chemotherapy, by 63% and reduced MRP1-protein levels by 70%. MRP1 siRNA loaded nanoparticles did not induce cytotoxicity in glioblastoma cells, but markedly reduced cell proliferation. In summary, the results demonstrated that non-cytotoxic cationic THCpSiNPs are promising vehicles for therapeutic siRNA delivery.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

S0389

Sigma-Aldrich

Saccharose, for molecular biology, ≥99.5% (GC)

S9378

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Saccharose, ≥99.5% (GC)

695068

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Fluorwasserstoffsäure, ACS reagent, 48%

S7903

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Saccharose, ≥99.5% (GC), BioXtra

G8540

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L-Glutamin, meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source

84097

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Saccharose, BioUltra, for molecular biology, ≥99.5% (HPLC)

P4170

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Propidiumjodid, ≥94.0% (HPLC)

G3126

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L-Glutamin, ReagentPlus^®, ≥99% (HPLC)

339261

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Fluorwasserstoffsäure, 48 wt. % in H₂O, ≥99.99% trace metals basis

201030

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Osmiumtetroxid, ReagentPlus^®, 99.8%

E8008

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Ethylendiamintetraessigsäure -Lösung, 0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture

S1888

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Saccharose, ≥99.5% (GC), BioReagent, suitable for cell culture, suitable for insect cell culture

251755

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Osmiumtetroxid -Lösung, 4 wt. % in H₂O

G5792

SAFC

L-Glutamin

S8501

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Saccharose, ≥99.5% (GC)

E6758

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Ethylendiamintetraessigsäure, anhydrous, crystalline, BioReagent, suitable for cell culture

431788

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Ethylendiamintetraessigsäure, 99.995% trace metals basis

S5391

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Saccharose, ≥99.5% (GC), Grade II, suitable for plant cell culture

75632

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Osmiumtetroxid -Lösung, suitable for electron microscopy, 4% in H₂O

208868

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Osmiumtetroxid -Lösung, 2.5 wt. % in tert-butanol

49419

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L-Glutamin, BioUltra, ≥99.5% (NT)

X100

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Triton^™ X-100, laboratory grade

T58203

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2,4,6-Tris(dimethylaminomethyl)phenol, 95%

31185

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D.E.R.^™ 332, used as embedding medium

S5390

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Saccharose, Grade I, ≥99% (GC), suitable for plant cell culture

E9884

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Ethylendiamintetraessigsäure, ACS reagent, 99.4-100.6%, powder

S3929

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Saccharose, meets USP testing specifications

443271

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Dodecenylbernsteinsäureanhydrid, Isomermischung, technical grade, 90%

419494

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Osmiumtetroxid, ACS reagent, ≥98.0%

75633

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Osmiumtetroxid -Lösung, suitable for electron microscopy, 2% in H₂O