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Merck

Preparation and evaluation of natural scaffold materials for kidney regenerative applications.

Methods in molecular biology (Clifton, N.J.) (2013-03-16)
Monica A Serban, Toyin A Knight, Richard G Payne
ZUSAMMENFASSUNG

Tissue engineering involves the concerted action of biomaterials, cells, and growth factors. Kidney -regeneration relies on the same combination of ingredients. Here, we describe an example of gelatin-based biomaterial preparation and its evaluation in the context of kidney biocompatibility and integration. This biomaterial manufacturing technique is simple, cost-effective, highly reproducible and the in vivo evaluation procedure highly informative on the biocompatibility and regenerative potential of the tested construct.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Gelatine -Lösung, Type B, 2% in H2O, tissue culture grade, BioReagent, suitable for cell culture
Sigma-Aldrich
Gelatine aus Schweinehaut, gel strength 300, Type A
Sigma-Aldrich
Gelatine aus Schweinehaut, powder, gel strength ~300 g Bloom, Type A, BioReagent, suitable for electrophoresis, suitable for cell culture
Sigma-Aldrich
Gelatine aus Haut von Kaltwasserfischen, solid
Sigma-Aldrich
Gelatine aus Rinderhaut, Type B, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
Gelatine aus Rinderhaut, gel strength ~225 g Bloom, Type B
Millipore
Gelatine aus Schweinehaut, medium gel strength, suitable for microbiology
Sigma-Aldrich
Gelatine aus Schweinehaut, gel strength ~175 g Bloom, Type A
Sigma-Aldrich
Gelatine aus Schweinehaut, gel strength 80-120 g Bloom, Type A
Sigma-Aldrich
Gelatine aus Rinderhaut, Type B
Sigma-Aldrich
Prionex® Highly purified Type A, aqueous solution
Sigma-Aldrich
Gelatine aus Schweinehaut, Type A, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture
Sigma-Aldrich
Gelatine, tested according to Ph. Eur.
Millipore
Gelatine aus Schweinehaut, suitable for microbiology, high gel strength
Millipore
Gelatine aus Schweinehaut, suitable for microbiology, low gel strength
Millipore
Gelatine aus Schweinehaut, suitable for microbiology, ultrahigh gel strength