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Merck

G2625

Sigma-Aldrich

Gelatine aus Schweinehaut

gel strength ~175 g Bloom, Type A

Synonym(e):

Teleostean gelatine

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About This Item

CAS-Nummer:
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352202
NACRES:
NA.61

Biologische Quelle

Porcine skin

Typ

Type A

Form

powder

Konzentration

65-85% (biuret)

Methode(n)

cell culture | mammalian: suitable

Löslichkeit

H2O: soluble 50 mg/mL

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Anwendung

This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, geltan can be used as a suspending and encapsulating agent, among other applications.

Komponenten

Gelatine ist ein heterogenes Gemisch aus wasserlöslichen Proteinen mit hoher mittlerer Molekularmasse, das in Kollagen vorhanden ist. Die Proteine werden mittels Sieden der relevanten Haut, Sehnen, Ligamente, Knochen usw. in Wasser extrahiert. Gelatine vom Typ A wird aus mit Säure aufgeschlossenem Gewebe gewonnen. Gelatine vom Typ B wird aus mit Kalklauge geäschertem Gewebe gewonnen.

Vorsicht

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Angaben zur Herstellung

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gel strength of 175.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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