ZRB3201

Anti-c-Kit/CD117 Antibody, clone 1I8 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(e): Anti-Cardiac Troponin T Antibody, clone 1G6 ZooMAb^® Rabbit Monoclonal, EC: 2.7.10.1, Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, Mast/stem cell growth factor receptor Kit, PBT, Piebald trait protein, Proto-oncogene c-Kit, SCFR, Tyrosine-protein kinase Kit, p145 c-kit, v-kit
• UNSPSC-Code: 12352203

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Rekombinant: expressed in HEK 293 cells
• Konjugat: unconjugated
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 1I8, recombinant monoclonal
• Beschreibung: recombinant, expressed in HEK 293 cells
• Produktlinie: ZooMAb^® learn more
• Form: lyophilized
• Mol-Gew.: calculated mol wt 109.86 kDa; observed mol wt ~150 kDa
• Aufgereinigt durch: using Protein A
• Speziesreaktivität: human
• Verpackung: antibody small pack of 25
• Grünere Alternativprodukt-Eigenschaften: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Erweiterte Validierung: recombinant expression; Learn more about Antibody Enhanced Validation
• Methode(n): affinity binding assay: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotyp: IgG
• Epitopsequenz: N-terminal
• Protein-ID-Hinterlegungsnummer: NP_000213.1
• UniProt-Hinterlegungsnummer: P10721
• Grünere Alternativprodukt-Kategorie: Aligned,
• Versandbedingung: ambient
• Lagertemp.: 2-8°C
• Angaben zum Gen: human ... KIT(3815)

Beschreibung

Allgemeine Beschreibung

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spezifität

Clone 1I8 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects c-Kit/CD117. It targets an epitope within 23 amino acids from the extracellular domain within the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 23 amino acids from the N-terminal region of human c-Kit/CD117.

Anwendung

Quality Control Testing

Evaluated by Western Blotting in TF-1 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected c-Kit/CD117 in TF-1 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected c-Kit/CD117 in Human brain tissue lysate.

Affinity Binding Assay: A representative lot of this antibody bound CD117/c-Kit peptide with a KD of 2.9 x 10-6 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected c-Kit/CD117 in Human gastrointestinal stromal tumor (GIST) tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Zielbeschreibung

Mast/stem cell growth factor receptor Kit (UniProt: P10721; also known as EC: 2.7.10.1, SCFR, Piebald trait protein, PBT, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog) is encoded by the KIT (also known as SCFR) gene (Gene ID: 3815) in human. c-Kit is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 26-524), a transmembrane domain (aa 525-545), and a cytoplasmic domain (aa 546-976). c-Kit has tyrosine protein kinase activity and serves as a cell-surface receptor for the stem cell factor (SCF)/KITLG. It plays a significant role in the regulation of cell survival, cell proliferation, stem cell maintenance, gametogenesis, and other functions. In response to SCF binding, c-Kit can activate several signaling pathways. In the absence of bound stem cell factor (SCF)

Physikalische Form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Rekonstituierung

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Lagerung und Haltbarkeit

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Rechtliche Hinweise

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable