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T1063

Sigma-Aldrich

Thrombin aus Humanplasma

lyophilized powder, ≥2800 NIH units/mg protein (E1%/280, 18.3)

Synonym(e):

Faktor IIa

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About This Item

CAS-Nummer:
9002-04-4
EC-Nummer:
3.4.21.5 (BRENDA, IUBMB)
EG-Nummer:
232-648-7
MDL-Nummer:
MFCD00082072
UNSPSC-Code:
12352204
NACRES:
NA.54

Form

lyophilized powder

Qualitätsniveau

200

Spezifische Aktivität

≥2800 NIH units/mg protein (E1%/280, 18.3)

Mol-Gew.

37.4 kDa

Verunreinigungen

HIV, hepatitis B and hepatitis C, tested negative

UniProt-Hinterlegungsnummer

P00734

Anwendung(en)

diagnostic assay manufacturing

Lagertemp.

−20°C

Angaben zum Gen

human ... F2(2147)

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Allgemeine Beschreibung

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.

Anwendung

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to evaluate the in vitro effect of low molecular weight heparin on thrombin generation in cirrhotic patients at different stages of liver disease.

Biochem./physiol. Wirkung

Serinprotease, die selektiv Arg-Gly-Bindungen in Fibrinogen spaltet, um Fibrin und Fibrinopeptide A und B zu bilden.

Einheitendefinition

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard

Physikalische Form

Lyophilisiert aus 0.02 M Bis/Tris Puffer, pH 6.5, 0.15 M NaCl und 0.1% PEG-8000

Hinweis zur Analyse

The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Sonstige Hinweise

Für weitere Informationen siehe thrombin at www.sigma-aldrich.com/enzymeexplorer.

Haftungsausschluss

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Inhibitor

Produkt-Nr.
Beschreibung
Preisangaben

Substrat

Produkt-Nr.
Beschreibung
Preisangaben

Piktogramme

Health hazardExclamation mark
GHS08,GHS07

Signalwort

Danger

Gefahreneinstufungen

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Zielorgane

Respiratory system

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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M Senzolo et al.
Journal of thrombosis and haemostasis : JTH, 10(9), 1823-1829 (2012-06-21)
Cirrhotic patients may present thrombotic complications that warrant anticoagulant therapy. However, the efficacy of low-molecular-weight heparin (LMWH) in this clinical setting is still unclear. To evaluate the in vitro effect of LMWH on thrombin generation (TG) in cirrhotic patients at
D David et al.
British journal of haematology, 113(3), 604-615 (2001-05-31)
In haemophilia A, the functional defect at the molecular level of most factor VIII (FVIII) missense mutations remains unknown. Site-directed mutagenesis of B domain-deleted FVIII cDNA (FVIIISQ) was used to introduce two mutations associated with severe cross-reacting material (CRM)-negative (FVIII-C329S)
Sooyeon Kim et al.
RSC advances, 8(37), 20822-20828 (2018-06-06)
We propose a new sensor design that maximizes fluorescence contrast, inspired by whispering-gallery mode lasing (WGM). Aptamer-modified glass microspheres (cf. 1-38 μm) and thrombin are used as a model sensory cavity and target protein, respectively. Two types of microsphere are
Giselle Barbosa-Lima et al.
Journal of leukocyte biology, 108(4), 1293-1306 (2020-07-15)
Dengue is characterized as one of the most important arthropod-borne human viral diseases, representing a public health problem. Increased activation of immune cells is involved in the progression of infection to severe forms. Recently, our group demonstrated the contribution of
Zhong Feng Gao et al.
Nature protocols, 15(2), 316-337 (2020-01-10)
Current visual biosensing methods, including colorimetric-based, fluorescence-based and chemiluminescence-based methods, are inappropriate for the hundreds of millions of people affected by color blindness and color weakness. Compared with these available methods, a droplet motion-based strategy might be a promising protocol
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Artikel

Thrombin Factor IIa

Thrombin Factor IIa is an endolytic serine protease that selectively cleaves the Arg--Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

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