SRP6472

Transferrin human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonym(e): DKFZp781D0156, PRO1557, PRO2086, TF
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in HEK 293 cells
• Markierung: 6-His tagged (C-terminus)
• Assay: ≥95% (SDS-PAGE)
• Form: lyophilized
• Mol-Gew.: calculated mol wt 76 kDa; observed mol wt 74 kDa by SDS-PAGE (DTT-reduced.)
• Verpackung: pkg of 100 μg; pkg of 20 μg
• Verunreinigungen: <1 EU/μg endotoxin (LAL test)
• UniProt-Hinterlegungsnummer: P02787
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: human ... TF(7018)

Beschreibung

Allgemeine Beschreibung

Transferrin is encoded by the TF gene that is mapped to human chromosome 3q22.1. It is a glycoprotein containing homologous iron-binding domains at N-terminal and C-terminal ends. Transferrin has a molecular weight of 80,000 Da and belongs to the transferrin superfamily, the members of which include lactoferrin, melanotransferrin, and ovotransferrin.

Anwendung

To supplement neurosphere culture medium and sphere differentiation medium.

Biochem./physiol. Wirkung

Transferrin is an iron-binding glycoprotein that primarily functions in the transport of plasma iron in vertebrates. It may also have a secondary function of facilitating cell proliferation. The binding of ferric iron to transferrin requires the presence of an anion, that functions as a bridge between the metal and the protein.

Physikalische Form

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH 7.0. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Rekonstituierung

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3