SRP6112

G6PD from E. coli

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Synonym(e): Glucose-6-phosphate 1-dehydrogenase, Zwf
• CAS-Nummer: 9001-40-5
• UNSPSC-Code: 12352204
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: Escherichia coli
• Rekombinant: expressed in E. coli
• Assay: ≥90% (SDS-PAGE)
• Form: liquid
• Mol-Gew.: 55.7 kDa (491 aa, 1-491 aa)
• Verpackung: pkg of 100 μg
• NCBI-Hinterlegungsnummer: P0AC53
• UniProt-Hinterlegungsnummer: P0AC53
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: Escherichia coli ... ZWF(7149716)

Beschreibung

Allgemeine Beschreibung

Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH.

Specific activity is 8-10 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP.

Biochem./physiol. Wirkung

Glucose-6-phosphate dehydrogenase (G6PD) converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produces NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency causes acute hemolytic anemia.

Physikalische Form

1 mg/mL solution in 50 mM MES pH 6.0, 0.1 mM PMSF, 2 mM EDTA, 0.5 mM DTT and 10% glycerol.

Angaben zur Herstellung

Centrifuge the vial prior to opening.

Sonstige Hinweise

MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable