SRP5190

HSP70, His tagged human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(e): HSP70-1, HSP72, HSPA1, HSPA1A, HSPA1B
• CAS-Nummer: 2071612-66-1
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in baculovirus infected Sf9 cells
• Assay: ≥70% (SDS-PAGE)
• Form: buffered aqueous glycerol solution
• Mol-Gew.: ~70 kDa
• NCBI-Hinterlegungsnummer: NM_005345
• Anwendung(en): cell analysis
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... HSPA1A(3303)

Beschreibung

Allgemeine Beschreibung

Heat shock protein 70 (HSP70) is a ubiquitous molecular chaperone. It comprises an N-terminal domain, nucleotide-binding domain (NBD), substrate-binding domain (SBD), and a C-terminal domain.

Anwendung

Heat shock protein 70 (HSP70), His tagged human has been used:

• to study the interaction between HSP70 and receptor of advanced glycation endproducts (RAGE) using protein proximity ligand assay (PLA)
• to facilitate the import of superoxide dismutase 2 (SOD2) into the mitochondria
• to study its role in muscle catabolism

Biochem./physiol. Wirkung

Heat shock protein 70 (HSP70) plays a role in the cellular protein folding and remodeling process. It is also involved in the translocation of polypeptides into the chloroplast, mitochondria, and endoplasmic reticulum. HSP70 facilitates dismantling of protein complexes and modulates protein activity. It also plays a role in guarding cells from proteotoxic stress, pathophysiological conditions, and organismal aging. HSP70 participates in the activation of several immune cells such as macrophages, natural killer (NK) cells, B lymphocytes, peripheral monocytes, and antigen-presenting cells (APCs).

Physikalische Form

Supplied in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

Angaben zur Herstellung

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Sicherheitshinweise

• Piktogramme: GHS08,GHS07
• Signalwort: Danger
• H-Sätze: H315,H319,H360D
• P-Sätze: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Gefahreneinstufungen: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Lagerklassenschlüssel: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable