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SRP5014

Sigma-Aldrich

CK1 δ, active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(e):

CK1D, CSNK1D, HCKID

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About This Item

UNSPSC-Code:
12352200

Rekombinant

expressed in baculovirus infected Sf9 cells

Produktlinie

PRECISIO® Kinase

Assay

≥70% (SDS-PAGE)

Form

buffered aqueous glycerol solution

Spezifische Aktivität

70-94 nmol/min·mg

Mol-Gew.

~72 kDa

NCBI-Hinterlegungsnummer

NM_001893

Versandbedingung

dry ice

Lagertemp.

−70°C

Angaben zum Gen

human ... CSNK1D(1453)

Allgemeine Beschreibung

Casein kinase 1 δ (CK1δ) is a member of the CK1 family of serine/threonine protein kinases which play an important role in diverse cell processes, including DNA replication and repair.

Biochem./physiol. Wirkung

CK1δ is a regulator of Yes-associated protein (YAP) transcription coactivator, which is a key regulator of organ size and a candidate human oncogene. CK1d is activated by CCK2R and it then phosphorylates polycystin 2 (PKD2) at Ser244. Phosphorylation of PKD2 leads to its nuclear accumulation and efficient phosphorylation of nuclear PKD2 substrates in human gastric cancer cells. CKId can phosphorylate in vitro deoxycytidine kinase (dCK), which is a key enzyme in the salvage of deoxynucleosides. Phosphorylation of dCK by CKId in vitro correlates with increased activity of this enzyme.

Physikalische Form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Angaben zur Herstellung

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Rechtliche Hinweise

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

A coordinated phosphorylation by Lats and CK1 regulates YAP stability through SCF?-TRCP
Bin Zhao
Genes & Development (2010)
The CK1 Family: Contribution to Cellular Stress Response and Its Role in Carcinogenesis
Uwe Knippschild
Frontiers in Oncology (2014)
Phosphorylation at Ser244 by CK1 determines nuclear localization and substrate targeting of PKD2
J. von Blume
The Embo Journal (2007)
Caroline Smal et al.
Archives of biochemistry and biophysics, 502(1), 44-52 (2010-07-20)
Deoxycytidine kinase (dCK) is a key enzyme in the salvage of deoxynucleosides and in the activation of several anticancer and antiviral nucleoside analogues. We recently showed that dCK was activated in vivo by phosphorylation of Ser-74. However, the protein kinase
J von Blume et al.
The EMBO journal, 26(22), 4619-4633 (2007-10-27)
Protein kinase D2 (PKD2), a member of the PKD family of serine/threonine kinases, is localized in various subcellular compartments including the nucleus where the kinase accumulates upon activation of G-protein-coupled receptors. We define three critical post-translational modifications required for nuclear

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