SRP3200

GDNF from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Synonym(e): ATF-1, Glial-Derived Neurotrophic Factor
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: mouse
• Rekombinant: expressed in E. coli
• Assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Form: lyophilized
• Wirksamkeit: ≤0.2 ng/mL
• Mol-Gew.: 15.1 kDa
• Verpackung: pkg of 10 μg
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: <0.1 EU/μg endotoxin, tested
• Farbe: white to off-white
• UniProt-Hinterlegungsnummer: P48540
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: mouse ... GDNF(14573)

Beschreibung

Allgemeine Beschreibung

GDNF (glial cell line derived neurotrophic factor) is a disulfide-linked homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. The functional murine GDNF ligand is a disulfide-linked homodimer, of two 15.1kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, one of which is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.

Anwendung

GDNF from mouse has been used for stimulating primary murine cultures of dorsal root ganglion (DRG) neurons.

GDNF from mouse has been used to study the role of NEDL2 (Nedd4-related E3 ubiquitin ligase-2) in GDNF/Ret signaling.

Biochem./physiol. Wirkung

GDNF (glial cell line derived neurotrophic factor) signals through a multicomponent receptor system, composed of a RET (receptor tyrosine kinase) and one of the four GFR-α (α1-α4) receptors (GDNF family receptors). GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Using Parkinson′s disease monkey model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. It might also be involved in the treatment of non-alcoholic fatty liver disease. GDNF is needed for proper spermatogonial stem cell survival and proliferation.

Sequenz

MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCESAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDNLV YHILRKHSAK RCGCI

Physikalische Form

Lyophilized from 10 mM Sodium Citrate.

Rekonstituierung

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Sonstige Hinweise

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable