SAB4200087

Anti-Dicer antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(e): Anti-Dicer1, Anti-Endoribonuclease Dicer, Anti-HERNA, Anti-Helicase with RNase motif
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~218 kDa
• Konzentration: ~1.0 mg/mL
• Methode(n): immunoprecipitation (IP): 2.5-5 μg using HeLa cell lysates; indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed HeLa cells; western blot: 3-6 μg/mL using HeLa cell lysates
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... DICER1(23405) ; mouse ... Dicer1(192119)

Beschreibung

Allgemeine Beschreibung

Dicer, also known as Dicer1, endoribonuclease Dicer, Helicase with RNase motif is a member of the RNase III family. It contains a N-terminal DEXH-box RNA helicase/ ATPase domain, followed by a domain of unknown function (DUF283), a Piwi/Argonaut/Zwill (PAZ ) domain, which anchors the 3′-end of RNA substrates.

Dicer1 is encoded by the gene mapped to human chromosome 14q32.13.

Spezifität

Anti-Dicer recognizes human Dicer.

Anwendung

Anti-Dicer antibody produced in rabbit has been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescent staining

Biochem./physiol. Wirkung

Dicer catalyzes the first step in the RNA interference (RNAi) pathway and initiates formation of the RNA-induced silencing complex (RISC). Dicer processes the dsRNA into small fragments called short interfering RNA (siRNA) or microRNA (miRNA) of typically 21-25 nucleotides long with a two-base overhang on the 3′. It helps to load these fragments into the RNA-induced silencing complex (RISC), which guides the sequence-specific silencing of mRNAs that contain complementary sequences by either enzymatically cleaving the mRNA or repressing its translation.

Mutation in the Dicer gene causes malignancies.

Physikalische Form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Lagerung und Haltbarkeit

Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable