S7570

SGK2 Active human

≥75% (SDS-PAGE), aqueous glycerol solution, recombinant, expressed in E. coli

• Synonym(e): Serum/Glucocorticoid-Regulated Kinase 2
• MDL-Nummer: MFCD07371188
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Rekombinant: expressed in E. coli
• Qualitätsniveau: 200
• Assay: ≥75% (SDS-PAGE)
• Form: aqueous glycerol solution
• Spezifische Aktivität: 100 units/mg protein (Bradford)
• Mol-Gew.: protein apparent mol wt ~66 kDa
• UniProt-Hinterlegungsnummer: Q9HBY8
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... SGK2(10110)

Beschreibung

Anwendung

Kinase activity is measured as the molar amount of phosphate incorporated into the Akt/SGK substrate peptide (RPRAATF), per minute per mg protein at 30 °C using a final concentration of 50 μM [³²P] ATP.

Biochem./physiol. Wirkung

SGK2 is a member of the serum- and glucocorticoid-induced kinases (SGK) which are serine-threonine kinases and belong to the "AGC" kinase subfamily, which includes protein kinases A, G, and C and has SGK2 enhances Na⁺/K⁺-ATPase activity in a variety of cells. In addition, SGK2 mimics the function of SGK1 and SGK3 and participates in the regulation of renal epithelial Na⁺ channel ENaC activity.

Einheitendefinition

One unit will incorporate one nanomole of phosphate into the AKT/SGK substrate peptide(RPRAATF) per minute at 30 °C and pH 7.2 using a final concentration of 50 μM [32P] ATP.

Physikalische Form

Supplied at 50 μL in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM dithiothreitol (DTT), 0.1 mM EGTA, and 30% glycerol.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable