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Merck

R8508

Sigma-Aldrich

Ribonukleinsäure, Transfer aus Backhefe (S. cerevisiae)

buffered aqueous solution

Synonym(e):

Transfer-RNA, tRNA

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About This Item

CAS-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352106
NACRES:
NA.55

Qualität

for molecular biology

Qualitätsniveau

Form

buffered aqueous solution

Konzentration

9-11 mg/mL

Fremdaktivität

DNase, Nickase, none detected

Versandbedingung

dry ice

Lagertemp.

−20°C

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Allgemeine Beschreibung

Transfer RNA (tRNA) is isolated from baker′s yeast.
Transfer RNA (tRNA) is a short nucleotide RNA chain, which has an L-shaped structure.

Anwendung

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) has been used to precipitate mRNA of denuded oocytes and for reverse transcription. It has also been used to seal magnetic beads in RNA pull-down assay of nasopharyngeal carcinoma (NPC) cells.
Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) is suitable for use as a carrier in nucleic acid purification and precipitation.

Biochem./physiol. Wirkung

Transfer RNA (tRNA) acts as an ‘adaptor′ molecule that translates the three-nucleotide codon sequence in the mRNA to the corresponding amino acid of that codon. It determines the genetic code by linking amino acids and nucleic acids. tRNA is involved in viral replication, bacterial cell wall biosynthesis, cell stress response, and animal behavior control. Aberrations in tRNA molecules are associated with several human diseases.

Komponenten

tRNA is provided in a solution in 10 mM Tris HCl (pH 7.4) in 1 mM EDTA.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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