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R7876

Sigma-Aldrich

Ribonukleinsäure, Transfer aus Weizenkeimen

Type V, 15-19 units/mg solid

Synonym(e):

Transfer-RNA, tRNA

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About This Item

CAS-Nummer:
9014-25-9
MDL-Nummer:
MFCD00132200
UNSPSC-Code:
12352106
NACRES:
NA.55

Qualität

for molecular biology

Qualitätsniveau

300

Typ

Type V

Form

lyophilized powder

Spezifische Aktivität

15-19 units/mg solid

Lagerbedingungen

desiccated

Löslichkeit

H2O: ~2 mg/mL, clear to hazy

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Wheat germ tRNA contains many different specific tRNA molecules.

Anwendung

Crude transfer RNA (tRNA), purified from wheat germ, contains many specific tRNA molecules that can be separated by different processes. Experiments for detailed investigation of structure and function using in vitro translation systems often require highly purified tRNA, which can be achieved by combinations of chromatographic separations and reversible biochemical modification of the tRNA. Wheat germ tRNA has been used for the purification of RNAse Z (tRNA 3′- processing endonucleases) from wheat germ. The purification included six steps with the most efficient one using an affinity column made of wheat germ tRNA, which the RNAse Z bound tightly.
Suitable for use as a carrier in nucleic acid purification and precipitation.

Komponenten

tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.

Einheitendefinition

One unit will yield an absorbance of 1.0 at 260 nm in 1.0 mL of water (1 cm light path).

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Sigma-Aldrich

H3393

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Spatial and temporal expression of the zebrafish genome by large-scale in situ hybridization screening.
Bernard Thisse et al.
Methods in cell biology, 77, 505-519 (2004-12-18)
Christine Thisse et al.
Nature protocols, 3(1), 59-69 (2008-01-15)
The in situ hybridization (ISH) technique allows the sites of expression of particular genes to be detected. This protocol describes ISH of digoxigenin-labeled antisense RNA probes to whole-mount zebrafish embryos. In our method, PCR-amplified sequence of a gene of interest
Christèle Maison et al.
Nature genetics, 30(3), 329-334 (2002-02-19)
Post-translational modification of histone tails is thought to modulate higher-order chromatin structure. Combinations of modifications including acetylation, phosphorylation and methylation have been proposed to provide marks recognized by specific proteins. This is exemplified, in both mammalian cells and fission yeast
Yana P Blokhina et al.
PLoS genetics, 15(1), e1007730-e1007730 (2019-01-18)
Meiosis is a cellular program that generates haploid gametes for sexual reproduction. While chromosome events that contribute to reducing ploidy (homologous chromosome pairing, synapsis, and recombination) are well conserved, their execution varies across species and even between sexes of the
Crystal E Fagan et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(24), 9716-9721 (2013-05-01)
After four decades of research aimed at understanding tRNA selection on the ribosome, the mechanism by which ribosomal ambiguity (ram) mutations promote miscoding remains unclear. Here, we present two X-ray crystal structures of the Thermus thermophilus 70S ribosome containing 16S
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