P3026

Pektolyase aus Aspergillus japonicus

lyophilized powder, ≥0.3 units/mg solid

• Synonym(e): Pectolytic enzyme
• EC-Nummer: 3.2.1.15 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00131813
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: Aspergillus sp. (A. japonicus)
• Qualitätsniveau: 200
• Form: lyophilized powder
• Spezifische Aktivität: ≥0.3 units/mg solid
• Mol-Gew.: 50 kDa
• Grünere Alternativprodukt-Eigenschaften: Waste Prevention; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• Grünere Alternativprodukt-Kategorie: , Enabling
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.

Pectolyase or pectin lyase is stable in the temperature range of 40-50°C and is a 50 kDa enzyme with an isoelectric pH of 3.8.

Anwendung

Pectolyase from Aspergillus japonicus has been used:

• in the lysis of chlorella cells for protoplast generation
• as component of enzyme digestion buffer for Arabidopsis meiotic chromosome isolation
• to induce reorganization of Arabidopsis root cap cell architecture

Used in plant protoplast preparation to digest cell wall prior to organelle isolation. It is used in complex carbohydrate analysis. Product P3026 is provided in a lyophilized powder containing lactose. Product P3026 has been used to digest Crassula root tips prior to meiotic analysis.

Zur Herstellung von Pflanzenprotoplast für den Zellwandabbau vor der organellen Isolation.

Biochem./physiol. Wirkung

Pectolyase catalyzes the eliminative cleavage of a-(1- 4)-Dgalacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-a-D-galact-4-enuronosyl groups at their non-reducing ends. It contain two types of pectinase, endopolygalacturonase, endo-pectin lyase and a maceration stimulating factora.

Pectolyase is used for protoplast isolation from plant cells. It also mediates ethylene hormone synthesis.

Sonstige Hinweise

Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor.

Für weitere Informationen siehe enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Einheitendefinition

1 U setzt 1.0 μmol Galacturonsäure aus Polygalacturonsäure pro Minute frei bei pH 5.5 und 25 °C.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)