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P0690

Sigma-Aldrich

Pektinase aus Aspergillus niger

suitable for plant cell culture, BioReagent, aqueous glycerol solution, ≥5 units/mg protein (Lowry)

Synonym(e):

Poly-(1,4-α-D-galacturonid)glycanohydrolase, Polygalacturonase -Lösung aus Aspergillus niger

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About This Item

CAS-Nummer:
9032-75-1
EC-Nummer:
3.2.1.15 (BRENDA, IUBMB)
EG-Nummer:
232-885-6
MDL-Nummer:
MFCD00131809
UNSPSC-Code:
12352200

Produktlinie

BioReagent

Form

aqueous glycerol solution

Spezifische Aktivität

≥5 units/mg protein (Lowry)

Methode(n)

cell culture | plant: suitable

Anwendung(en)

agriculture

Lagertemp.

2-8°C

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Anwendung

Pectinase P0690 is a natural pectinase (polygalacturonase) produced by Aspergillus niger. This enzyme breaks down pectin found in the middle lamella of plant cell walls. Pectinase helps facilitate the maceration of plant materials and extraction processes including organelle preparation.
Wird bei der Vorbereitung von Pflanzenprotoplasten für den Zellwandaufschluss vor der Isolation von Organellen eingesetzt.

Einheitendefinition

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C.

Physikalische Form

Solution in 40% glycerol

Analysenzertifikate (COA)

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Sina Pricelius et al.
Journal of agricultural and food chemistry, 57(3), 1006-1012 (2009-02-05)
Attractive color is one of the most important sensory characteristics of fruit and berry products, and elderberry juice is widely used as natural colorant. When pectinase preparations were used in the production of elderberry juice for clarification, a concomitant decrease
Zeratsion Abera Desta et al.
Scientific reports, 9(1), 17028-17028 (2019-11-21)
Field cress (Lepidium campestre L.), despite its potential as a sustainable alternative oilseed plant, has been underutilized, and no prior attempts to characterize the genome at the genetic or molecular cytogenetic level have been conducted. Genetic maps are the foundation
Vincenzo Lionetti et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(2), 616-621 (2010-01-19)
Plant cell walls represent an abundant, renewable source of biofuel and other useful products. The major bottleneck for the industrial scale-up of their conversion to simple sugars (saccharification), to be subsequently converted by microorganisms into ethanol or other products, is
Sara Posé et al.
Journal of experimental botany, 64(12), 3803-3815 (2013-07-23)
Antisense-mediated down-regulation of the fruit-specific polygalacturonase (PG) gene FaPG1 in strawberries (Fragaria×ananassa Duch.) has been previously demonstrated to reduce fruit softening and to extend post-harvest shelf life, despite the low PG activity detected in this fruit. The improved fruit traits
Mi Wei et al.
Bioresource technology, 146, 549-555 (2013-08-27)
A novel method utilizing microbial treatment for cleaner production of diosgenin from Dioscorea zingiberensis C.H. Wright (DZW) was presented. A new Bacillus pumilus HR19, which has the great ability to secrete pectinase, was screened and applied in the microbial treatment.
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