P0050

Anti-Peroxiredoxin 6 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-1-Cys peroxiredoxin; acidic calcium-independent phospholipase A2, Anti-Antioxidant protein 2, Anti-NSGPx, Anti-Non-selenium glutathione peroxidase, AOP2, Anti-P29, Anti-PRDX6, Anti-PRX, Anti-aiPLA2
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~25 kDa
• Speziesreaktivität: human, mouse, rat
• Verpackung: antibody small pack of 25 μL
• Konzentration: ~1.0 mg/mL
• Methode(n): immunoprecipitation (IP): 5-10 μg using lysate of mouse brain; western blot: 2-5 μg/mL using whole extract of human HeLa cells; western blot: 5-10 μg/mL using whole extract of rat liver
• UniProt-Hinterlegungsnummer: P30041
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... PRDX6(9588) ; mouse ... Prdx6(11758) ; rat ... Prdx6(94167)

Beschreibung

Allgemeine Beschreibung

Peroxiredoxin 6 (1-Cys peroxiredoxin), is a member of the thiol-specific antioxidant peroxiredoxin family. Six mammalian peroxiredoxins have been identified. Peroxiredoxin 6 is the only one that contains a single redox-active cysteine and uses glutathione to catalyze the reduction of H₂O₂ and other organic peroxides. It is expressed in all tissues with the highest levels in the lung.

Anwendung

The antibody has been used in several immunochemical techniques including immunoblotting and immunoprecipitation.

Biochem./physiol. Wirkung

Peroxiredoxins are peroxidases, with the use of reducing equivalents provided by thiol-containing proteins that reduce hydrogen peroxide (H₂O₂) to water and alkyl hydroperoxides to alcohol. Peroxiredoxin 6 is a bifunctional enzyme with peroxidase and phospholipase A2 activities. Overexpression of peroxiredoxin 6 in cells protects them against oxidative damage, whereas knockdown of this enzyme results in oxidative stress and apoptosis. The phospholipase A2 activity plays an important role in surfactant homeostasis. Peroxiredoxin 6 is a major antioxidant enzyme which functions in antioxidant defense and lung phospholipid metabolism.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)