N9414

Anti-NUCB2 (N-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-Calnuc, Anti-NEFA, Anti-Nef, Anti-Nesfatin, Anti-Nucleobindin 2, Anti-p54
• UNSPSC-Code: 12352203
• NACRES: NA.44
• Konjugat: unconjugated
• application: IF; WB
• Klon: polyclonal
• Speziesreaktivität: rat, mouse
• citations: 9
• Methode(n): indirect immunofluorescence: 1-2 μg/mL using Rat2 cells; western blot: 1.5-3.0 μg/mL using Rat2 cell lysates; western blot: 2-4 μg/mL using mouse testis extracts (S1 fraction)

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~54 kDa
• Speziesreaktivität: rat, mouse
• Konzentration: ~1.5 mg/mL
• Methode(n): indirect immunofluorescence: 1-2 μg/mL using Rat2 cells; western blot: 1.5-3.0 μg/mL using Rat2 cell lysates; western blot: 2-4 μg/mL using mouse testis extracts (S1 fraction)
• UniProt-Hinterlegungsnummer: P81117
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: rat ... Nucb2(59295)

Beschreibung

Allgemeine Beschreibung

NUCB2 (N-terminal) (nucleobindin 2, also known as NEFA, p54) belongs to the nucleobindin family of EF-hand Ca²⁺-binding proteins.

Nucleobindin 2 (NUCB2), contain multiple functional domains, including a Leu/Ile-rich region, a putative nuclear localization signal, a DNA binding domain, two Ca²⁺ -binding EF-hand motifs, and a leucine zipper region. NUCB2 is widely distributed and is localized to the Golgi apparatus. Human NUCB2 has high homology (62% sequence identity) with human NUCB1, an additional member of the nucleobindin family, although they are encoded by two separate genes.

Anwendung

Anti-NUCB2 (N-terminal) antibody produced in rabbit has been used in:

• immunofluorescence
• western blotting
• immunohistochemistry

Biochem./physiol. Wirkung

Nucleobindin 2 (NUCB2) is implicated in various functions, including Ca²⁺ homeostasis, hypothalamic regulation of feeding, and tumor necrosis factor (TNF)-receptor-1 (TNFR1) shedding. NUCB2 has been shown to interact with both necdin and endoplasmic reticulum aminopeptidase 1 (ARTS-1), both through the EF-hand motif. NUCB2-ARTS-1 complex is required for the proteolytic cleavage and release of TNFR1 to the extracellular compartment. NUCB2 has been recently identified as a novel satiety molecule in the hypothalamus, associated with melanocortin signaling in the central nervous system. NUCB2 and its N-terminal 82-amino acid cleavage fragment, termed nesfatin-1, have been shown to induce suppression of food-intake in rats.

Physikalische Form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable