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Merck

N6655

Sigma-Aldrich

Anti-Nerve Growth Factor 2.5S antibody produced in rabbit

fractionated antiserum, buffered aqueous solution

Synonym(e):

Anti-NGF-2.5S

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About This Item

EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
51111800
NACRES:
NA.41

Biologische Quelle

rabbit

Qualitätsniveau

Konjugat

unconjugated

Antikörperform

fractionated antiserum

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Speziesreaktivität

mouse, human

Methode(n)

Ouchterlony double diffusion: suitable
western blot: 1:2,000

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... NGF(4803)
mouse ... Ngf(18049)

Allgemeine Beschreibung

Nerve growth factor (NGF) belongs to the family of neurotrophic factors. It is located at 1p13.2 on the human chromosome. There are two molecular forms of NGF, 7S NGF and 2.5S NGF, that have identical neurotrophic effect on neurons.

Spezifität

May be used in monitoring for NGF (2.5S, β and 7S).

Immunogen

NGF-2.5S from male mouse submaxillary glands.

Anwendung

Anti-Nerve Growth Factor 2.5S antibody may be used for immunoblotting and dot blot at a minimum antibody working dilution of 1:2000. Antibody dilution of 1:1000 was used for immunoblotting using extracts of nucleus raphe magus tissue of rats. The antibody was used for immunofluorescence of mouse astrocytes and for neutralization assays.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Western Blotting (1 paper)

Biochem./physiol. Wirkung

Mutations in this gene leads to hereditary sensory and autonomic neuropathies class five (HSAN-V).

Physikalische Form

Solution in phosphate buffered saline.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Kunden haben sich ebenfalls angesehen

7S nerve growth factor has different biological activity from 2.5 S nerve growth factor in vitro
Shao N, et al.
Brain Research, 609(1-2), 338-340 (1993)
N Shao et al.
Brain research, 609(1-2), 338-340 (1993-04-23)
It is considered that two molecular forms of nerve growth factor (NGF), that is 7S NGF and 2.5S NGF have the identical neurotrophic effect on neurons. We now report that 7S NGF has different biological activities from 2.5S NGF in
Liang-Wei Chen et al.
The Journal of comparative neurology, 497(6), 898-909 (2006-06-28)
To address the hypothesis that reactive astrocytes in the basal ganglia of an animal model of Parkinson's disease serve neurotrophic roles, we studied the expression pattern of neurotrophic factors in the basal ganglia of C57/Bl mice that had been treated
Joshua S Barker et al.
Journal of toxicology and environmental health. Part A, 78(6), 397-407 (2015-03-04)
Previous studies demonstrated that interleukin-1β (IL-1β) and nerve growth factor (NGF) increase synthesis of substance P (SP) in airway neurons both after ozone (O3) exposure and by direct application. It was postulated that NGF mediates O3-induced IL-1β effects on SP.
New protein fold revealed by a 2.3-
McDonald N Q, et al.
Nature, 354(6352), 411-411 (1991)

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