N5415

Nerve Growth Factor-ß human

recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

• Synonym(e): β-NGF, BNGF, NGF-β, NGF-beta
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Biologische Quelle: human
• Qualitätsniveau: 200
• Rekombinant: expressed in HEK 293 cells
• Assay: ≥95% (SDS-PAGE)
• Form: lyophilized powder
• Wirksamkeit: ≤16 ng/mL ED₅₀/EC₅₀
• Mol-Gew.: protein calculated mol wt 13.5 kDa (Monomer)
• Verpackung: pkg of 10 μg; pkg of 100 μg
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: ≤1 EU/μg Endotoxin (β-NGF)
• UniProt-Hinterlegungsnummer: P01138
• Lagertemp.: −20°C
• Angaben zum Gen: human ... NGF(4803)

Beschreibung

Allgemeine Beschreibung

NGF-β gene is mapped to human chromosome 1p13.2.

Recombinant human β-NGF is expressed in human 293 cells as a homodimer (non-disulfide bonded) of 120 amino acids, with a calculated molecular weight of 13.5 kDa (monomer). Nerve growth factor β (β-NGF) is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. The production of natural β-NGF occurs in epithelial cells, and the pituitary gland.

Biochem./physiol. Wirkung

A mutation in the NGF-β gene is implicated in the loss of pain perception.

Nerve growth factor β (β-NGF) is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF promotes the survival and cholinergic phenotype of basal forebrain cholinergic neurons and stimulates neurite outgrowth of embryonic trigeminal motor neurons in vitro.

Vorsicht

The product retains activity for at least 2 years as supplied. After initial thawing it is recommended to store the protein in working aliquots at -20 °C.

Physikalische Form

This product is lyophilized from PBS.

Angaben zur Herstellung

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile DDW containing 0.1% endotoxin-free recombinant human serum albumin, to a final concentration of 100 μg/ml.

Lagerung und Haltbarkeit

Store product at –20 °C. The product retains activity for at least 2 years as supplied. After initial thawing it is recommended to store the protein in working aliquots at –20 °C.

Hinweis zur Analyse

The specific activity is determined by the dose dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Haftungsausschluss

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable