N3254

4-Nitrophenylphosphat Di-(tris)-salz

phosphatase substrate, ≥97% (enzymatic), powder

• Synonym(e): 4-Nitrophenylphosphat Bis-(Tris)-salz
• Lineare Formel: (C₆ H₄ N O₆ P)( C₄ H₁₂ N O₃)₂
• CAS-Nummer: 68189-42-4
• Molekulargewicht: 461.36
• Beilstein: 1578142
• EG-Nummer: 269-198-6
• MDL-Nummer: MFCD00037009
• UNSPSC-Code: 12352204
• PubChem Substanz-ID: 24897633
• NACRES: NA.83

Eigenschaften

• product name: 4-Nitrophenylphosphat Di-(tris)-salz, phosphatase substrate
• Assay: ≥97% (enzymatic)
• Form: powder
• Löslichkeit: water: 50 mg/mL, clear, colorless to light yellow
• Lagertemp.: −20°C
• SMILES String: NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O
• InChI: 1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2
• InChIKey: XXAXKCWOTRABOW-UHFFFAOYSA-N

Beschreibung

Anwendung

4-Nitrophenyl phosphate di(tris) salt has been used:

• as a substrate to quantify tartrate-resistant acid phosphatase (TRAP) activity in cell lysates
• to determine phosphatase activity using native polyacrylamide gel electrophoresis (PAGE)
• for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA)

Biochem./physiol. Wirkung

p-Nitrophenyl Phosphate (pNPP) is a preferred substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures, because of its high sensitivity. This substrate produces a soluble yellow end-product and can be read spectrophotometrically at 405 nm.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)