MTOX1097

MRP7 Knockout Caco-2 Cells

one vial

• UNSPSC-Code: 12352200
• NACRES: NA.81

Eigenschaften

• Biologische Quelle: human male colorectal tissue (Source Disease: colon adenocarcinoma )
• Qualitätsniveau: 200
• Form: liquid
• Methode(n): drug transporter assay: suitable; permeability assay: suitable
• Anwendung(en): ADME/TOX
• Lagertemp.: −196°C
• Angaben zum Gen: human ... ABCC10(89845)

Beschreibung

Allgemeine Beschreibung

The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MRP6 knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCC10 (MRP6) efflux transporter.

Anwendung

The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:

Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases

Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes

Caco-2 Transporter Knockout Cell Based Assays

Leistungsmerkmale und Vorteile

The Caco-2 subclone C2BBe1 cells are ideal for transporter analysis as they express multiple transporters, are human derived, and grow in a homogenous monolayer that forms tight junctions necessary for efflux ratio analysis. Other benefits include:

• A functional knockout of the MRP6 gene eliminates the reliance on chemical inhibitors to determine if a compound is an MRP6 substrate
• The vial format enables the MRP6 knockout cells to be included in standard drug transporter protocols
• Human assay with no interference from animal inhibitors
• Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality

Rechtliche Hinweise

ADME/Tox Cell Lines License

Haftungsausschluss

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable