MSP01

Membran-Gerüstprotein 1D1

recombinant, expressed in E. coli, buffered aqueous solution

• Synonym(e): MSP1D1, MSP1T2
• UNSPSC-Code: 12352200

Eigenschaften

• Rekombinant: expressed in E. coli
• Qualitätsniveau: 200
• Assay: ≥90% (SDS-GE)
• Form: buffered aqueous solution
• Versandbedingung: ambient
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Nanodiscs are non-covalent structures that contain a phospholipid bilayer and a membrane scaffold protein (MSP), a genetically engineered protein, which mimics the function of Apolipoprotein A-1 (ApoA-1). The first MSP, MSP1, was engineered with its sequence based on the sequence of A-1, but without the globular N-terminal domain of native A-1. The Membrane Scaffold Protein 1D1 (MSP1D1) variant of MSP1 deletes the first 11 amino acids in the Helix 1 portion (referred to as “H0.5” in the accompanying figure) of the original MSP1 sequence.

Anwendung

Das Nanodisc-System wird zum Integrieren einer Vielzahl von Proteinen, einschließlich GPCRs, P450s, Bacteriorhodopsin, Gerinnungsfaktoren, Choleratoxin, TAR-Rezeptor und Aromatase, eingesetzt.

Nanodiscs mit löslichen Lipiddoppelschichten werden in vielen Anwendungen eingesetzt, um Membranproteine in wässrigen Lösungen in einer naturgetreuen Doppelschicht, in der sie ihre Monodispersität und Aktivität aufrechterhalten, löslich zu machen. Die wichtigste Komponente der Nanodiscs ist der umschließende amphipathische helikale Proteingürtel (Membran-Gerüstprotein).

For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page.

Biochem./physiol. Wirkung

Zur Herstellung von Nanodiscs mit einem Durchmesser von ~9,7 nm

Rechtliche Hinweise

Die Nanodisc-Technologie und viele Anwendungen davon sind unter den folgenden Patenten der University of Illinois geschützt.

• 7,691,414 Membrane scaffold proteins
• 7,662,410 Membrane scaffold proteins and embedded membrane proteins
• 7,622,437 Tissue factor compositions and methods
• 7,592,008 Membrane scaffold proteins
• 7,575,763 Membrane scaffold proteins and tethered membrane proteins
• 7,083,958 Membrane scaffold proteins
• 7,048,949 Membrane scaffold proteins

Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois.

• 7,691,414 Membrane scaffold proteins
• 7,662,410 Membrane scaffold proteins and embedded membrane proteins
• 7,622,437 Tissue factor compositions and methods
• 7,592,008 Membrane scaffold proteins
• 7,575,763 Membrane scaffold proteins and tethered membrane proteins
• 7,083,958 Membrane scaffold proteins
• 7,048,949 Membrane scaffold proteins

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable