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MAK178
Enolase Activity Assay Kit
Sufficient for 100 Colorimetric or Fluorometric tests
Synonym(e):
Enolase Detection Kit
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About This Item
UNSPSC-Code:
12161503
NACRES:
NA.84
Empfohlene Produkte
Nachweisverfahren
colorimetric
fluorometric
Relevante Krankheit(en)
immunological diseases; cardiovascular diseases
Lagertemp.
−20°C
Angaben zum Gen
human ... EBI4(387712) , ENO1(2023) , ENO2(2026)
mouse ... EBI4(226265) , ENO1(13806) , ENO2(13807)
rat ... EBI4(292138) , ENO1(24333) , ENO2(24334)
Allgemeine Beschreibung
Enolase is a multifunctional glycolytic enzyme that catalyzes the conversion of D-2-phosphoglycerate to phospho(enol)pyruvate (PEP) and water. It can function as a plasminogen receptor in endothelial, epithelial, and hematopoietic cells and hence, may be involved in fibrinolytic and intravascular systems. It is also known to act as a heat shock protein, which may have implications in transcriptional and pathological functions. Enolase has been implicated in autoimmune and systemic diseases. Furthermore; serum neuron-specific enolase is known to function as a predictor of patient outcome post cardiac arrest. Thus enolase assays can be used for studying cellular functions like carbohydrate metabolism, transcription and other pathophysiological processes.
Anwendung
Enolase Activity Assay Kit has been used to measure enolase activity.
Eignung
Suitable for the detection of enolase activity in biological samples.
Prinzip
The Enolase activity is determined by a coupled enzyme assay in which D-2-phosphoglycerate is converted to PEP, resulting in the formation of an intermediate that reacts with a peroxidase substrate, generating a colorimetric (570 nm) or fluorometric (λex = 535/λem = 587 nm) product proportional to the enolase activity present. One milliunit of enolase is the amount of enzyme that will generate 1.0 nmole of H2O2 per minute at pH 7.2 at 25 °C.
Nur Kit-Komponenten
Produkt-Nr.
Beschreibung
- Enolase Assay Buffer
- Peroxidase Substrate, in DMSO
- Enolase Substrate Mix
- Enolase Converter
- Enolase Developer
- Enolase Positive Control
- Hydrogen Peroxide Standard, 0.88 M
H-Sätze
P-Sätze
Gefahreneinstufungen
Aquatic Chronic 3
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 3
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