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MAK049

Sigma-Aldrich

Phosphatidylcholine Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(e):

Phosphatidylcholine Test Kit

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About This Item

UNSPSC-Code:
12161503
NACRES:
NA.84

Verwendung

sufficient for 100 colorimetric or fluorometric tests

Nachweisverfahren

colorimetric
fluorometric

Relevante Krankheit(en)

neurological disorders; gastrointestinal diseases; cancer

Lagertemp.

−20°C

Allgemeine Beschreibung

Phosphatidylcholine (PC) accounts for more than 50% of the phospholipids composing mammalian plasma membranes and is particularly enriched in the extracellular leaflet. PC is synthesized in the liver and is required for lipoprotein assembly and secretion. PC is cleaved by phospholipase D, which hydrolyses the choline head, producing the signaling intermediate phosphatidic acid.

Anwendung

Phosphatidylcholine Assay Kit has been used to determine the concentration of phosphatidylcholine in samples.

Eignung

Suitable for the measurement of phosphatidylcholine in a variety of biological samples

Prinzip

In this assay, PC concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the PC present.

Piktogramme

Health hazard
GHS08

Signalwort

Danger

H-Sätze

H334

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

10 - Combustible liquids

Flammpunkt (°F)

188.6 °F - closed cup

Flammpunkt (°C)

87 °C - closed cup

Analysenzertifikate (COA)

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USP

USP

1535755

Phosphatidylcholin (Soja)

Phosphorus standard solution 0.65 mM (phosphorous)

Supelco

P3869

Phosphorus standard solution

L-α-Phosphatidylcholin egg yolk, Type XVI-E, ≥99% (TLC), lyophilized powder

Sigma-Aldrich

P3556

L-α-Phosphatidylcholin

vibrant-m

Y0001905

Phosphatidylcholin aus Eigelb

Iron depletion induces hepatic secretion of biliary lipids and glutathione in rats
Alena P, et al.
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1862(12), 1469-1480 (2017)
Riboflavin attenuates myocardial injury via LSD1-mediated crosstalk between phospholipid metabolism and histone methylation in mice with experimental myocardial infarction.
Peng W, et al.
Journal of Molecular and Cellular Cardiology, 115, 115-129 (2018)
Copper-64 labeled liposomes for imaging bone marrow.
Sang-gyu L, et al.
Nuclear Medicine and Biology, 43(12), 781-787 (2016)
Sang-Gyu Lee et al.
Nuclear medicine and biology, 43(12), 781-787 (2016-10-04)
Bone marrow is the soft tissue compartment inside the bones made up of hematopoietic cells, adipocytes, stromal cells, phagocytic cells, stem cells, and sinusoids. While [18F]-FLT has been utilized to image proliferative marrow, to date, there are no reports of
Mun-Teng Wong et al.
Journal of virology, 90(20), 9075-9095 (2016-08-05)
Hepatitis C virus (HCV) infection reorganizes cellular membranes to create an active viral replication site named the membranous web (MW). The role that human choline kinase-α (hCKα) plays in HCV replication remains elusive. Here, we first showed that hCKα activity

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