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MAK137

Sigma-Aldrich

Phospholipase D Activity Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(e):

PLD Activity Assay Kit

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About This Item

UNSPSC-Code:
12161503
NACRES:
NA.84

Verwendung

sufficient for 100 colorimetric or fluorometric tests

Nachweisverfahren

colorimetric
fluorometric

Relevante Krankheit(en)

cancer

Lagertemp.

−20°C

Angaben zum Gen

human ... PLD1(5337) , PLD2(5338) , PLD3(23646) , PLD4(122618) , PLD5(200150) , PLD6(201164)
mouse ... PLD1(18805) , PLD2(18806) , PLD3(18807) , PLD4(104759) , PLD5(319455) , PLD6(194908)
rat ... PLD1(25096) , PLD2(25097) , PLD3(361527) , PLD4(362792) , PLD5(289270) , PLD6(287366)

Verwandte Kategorien

Allgemeine Beschreibung

Phospholipase D (PLD) catalyzes the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and choline. It is ubiquitously expressed and is implicated in immune function and cancer inhibition. Abnormalities in PLD expression have been associated with human cancers such as breast, renal, gastric and colorectal cancer.

Anwendung

Phospholipase D (PLD) Activity Assay Kit has been used to measure PLD activity in the liver and lysosomal fractions of the mice liver.
Suitable for the detection of phospholipase D activity in biological samples. This kit can be used to study the effects of various compounds on phospholipase D activity.

Leistungsmerkmale und Vorteile

Compatible with high-throughput handling systems.

Eignung

Suitable for the quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism.

Prinzip

In this assay, PLD hydrolyzes phosphatidylcholine to choline, which is determined using choline oxidase resulting in a colorimetric (570 nm)/fluorometric (λex = 530/λem = 585 nm) product, proportional to the PLD activity in the sample. Unit definition: one unit of PLD catalyzes the formation of 1 mmole choline per minute under the assay conditions (pH 7.4).

Piktogramme

Health hazard
GHS08

Signalwort

Danger

H-Sätze

H334,H412

Gefahreneinstufungen

Aquatic Chronic 3 - Resp. Sens. 1

Lagerklassenschlüssel

10 - Combustible liquids

Flammpunkt (°F)

188.6 °F - closed cup

Flammpunkt (°C)

87 °C - closed cup

Analysenzertifikate (COA)

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Design of isoform-selective phospholipase D inhibitors that modulate cancer cell invasiveness.
Scott S A, et al.
Nature Chemical Biology, 5(2), 108-108 (2009)
Przemysław Bernat et al.
Journal of plant physiology, 229, 158-163 (2018-08-11)
2,4-dichlorophenoxyacetic acid (2,4-D) is among the most commonly used herbicides applied for weed control during wheat cultivation. However, its application could affect wheat growth. The present study investigates the effect of the ascomycetous fungus Trichoderma harzianum on lipid peroxidation, phospholipids
Walid El Kayal et al.
Horticulture research, 4, 17042-17042 (2017-11-09)
Raspberry (Rubus spp.) is an economically important crop with a restricted growing season and very limited fruit shelf-life due to its extreme tenderness. In order to prolong its shelf life, an aqueous composition containing hexanal as the key active ingredient
Phospholipase D.
Su W and Michael A F
Handbook of Cell Signaling, 1167-1176 (2009)
Jeong Kuk Park et al.
IUCrJ, 6(Pt 4), 729-739 (2019-07-19)
Ependymin was first discovered as a predominant protein in brain extracellular fluid in fish and was suggested to be involved in functions mostly related to learning and memory. Orthologous proteins to ependymin called ependymin-related proteins (EPDRs) have been found to
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