M3817

MegaCell^™ RPMI-1640 Medium

without L-glutamine, liquid, sterile-filtered, suitable for cell culture

• UNSPSC-Code: 12352207
• NACRES: NA.75

Eigenschaften

• Qualitätsniveau: 500
• Sterilität: sterile-filtered
• Form: liquid
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: endotoxin, tested
• Komponenten: L-glutamine: no
• Versandbedingung: ambient
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

MegaCell^™ is a line of versatile media, formulated to significantly reduce the amount of serum required for cultivating mammalian cells in vitro. It is effective as a basal formulation for the growth and maintenance of adherent or suspension cell lines. When supplemented with 3% fetal bovine serum (FBS), MegaCell supports proliferative rates and maximal cell densities comparable, and in some cases superior, to the conventional basal formulation supplemented with 10% fetal bovine serum.

Anwendung

MegaCell^™ RPMI-1640 Medium has been used:

• as a component of the TSC medium for culturing trophoblast stem cells (TSC)
• as a component of 25 ng/ml FGF4 and 1 g/ml heparin (TSF4H medium) for culturing TSCs
• as a component of embryo-like structures (ETS) embryo medium and TS cell medium for culturing embryonic stem cells (ESCs) and TSCs

Rekonstituierung

Supplement with 4 mM L-glutamine (20 ml of 200 mM solution, G7513)

Rechtliche Hinweise

Sigma-Aldrich uses the MegaCell trademark pursuant to an agreement with Promega Corporation.

MegaCell is a trademark of Promega Corp.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable