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M1018

Sigma-Aldrich

Minimum Essential Medium Eagle

With Hanks′ salts, L-glutamine and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

Synonym(e):

MEM

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About This Item

UNSPSC-Code:
12352207
NACRES:
NA.75

Form

powder

Methode(n)

cell culture | mammalian: suitable

Komponenten

phenol red: yes
NaHCO3: no
HEPES: no
sodium pyruvate: no
Hanks’ salts (2% CO2): yes
L-glutamine: yes

Versandbedingung

ambient

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Modification of the original MEM formulation. Earle′s salts have been replaced with Hanks′ salts. Since the bicarbonate concentration is substantially lower, this medium has a substantially reduced buffering capacity. It contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.

Menge

Formulated to contain 10.7 grams of powder per liter of medium.

Rekonstituierung

Supplement with 0.35 g/L sodium bicarbonate.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Claudia Penna et al.
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Nguyen T K Vo et al.
Developmental and comparative immunology, 92, 140-149 (2018-11-20)
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Tadashi Yoshida et al.
Cellular signalling, 73, 109690-109690 (2020-06-20)
Minocycline, an FDA-approved second-generation semisynthetic tetracycline, exerts antioxidant, anti-apoptotic and anti-inflammatory effects, independent of its antimicrobial properties. Interleukin (IL)-17A is an immune and inflammatory mediator, and its sustained induction is associated with various cardiovascular diseases. Here we investigated (i) whether
Melina J Sedano et al.
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Hypoxanthine phosphoribosyl transferase 1 (HPRT1) is traditionally believed to be a housekeeping gene; however, recent reports suggest that it is upregulated in several cancers and is associated with clinical outcomes. HPRT1 is located on chromosome X and encodes the HPRT
Mario G Pavez-Giani et al.
International journal of molecular sciences, 22(9) (2021-05-01)
ATPase inhibitory factor-1 (IF1) preserves cellular ATP under conditions of respiratory collapse, yet the function of IF1 under normal respiring conditions is unresolved. We tested the hypothesis that IF1 promotes mitochondrial dysfunction and pathological cardiomyocyte hypertrophy in the context of
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Protokolle

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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