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M0644

Sigma-Aldrich

Minimum Essential Medium Eagle

Alpha Modification, with L-glutamine, ribonucleosides and deoxyribonucleosides, without sodium bicarbonate, powder, suitable for cell culture

Synonym(e):

MEM

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About This Item

UNSPSC-Code:
12352207
NACRES:
NA.75

Qualitätsniveau

500

Form

powder

Methode(n)

cell culture | mammalian: suitable

Komponenten

NaHCO3: no
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: yes

Versandbedingung

ambient

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Minimum Essential Medium (MEM) is a synthetic cell culture media developed by Harry Eagle. This medium has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to this formulation that incorporates either Hanks′ or Earle′s salts has broadened the usage of this medium. The α modification of MEM with Earle′s balanced salts also known as αMEM, contains non-essential amino acids, sodium pyruvate, and additional vitamins.

Anwendung

Minimum essential medium Eagle has been used to:

  • maintain natural killer (NK) cell lines
  • culture Leishmania donovani strains
  • culture epidermal neural crest stem cells (EPI-NCSCs) from the bulge of rat hair follicles

Menge

Formulated to contain 10.1 grams of powder per liter of medium.

Rekonstituierung

Supplement with 2.2 g/L sodium bicarbonate.

Ergänzung

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Piktogramme

Exclamation mark
GHS07

Signalwort

Warning

H-Sätze

H317

Gefahreneinstufungen

Skin Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

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Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Protokolle

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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