L4669

Anti-Lysyl Oxidase (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-LOX, Anti-Protein lysine 6-oxidase
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~50 kDa
• Speziesreaktivität: human
• Erweiterte Validierung: recombinant expression; Learn more about Antibody Enhanced Validation
• Konzentration: ~1 mg/mL
• Methode(n): western blot: 1.5-3.0 mg/mL using HEK-293T cellls expressing human LOX
• UniProt-Hinterlegungsnummer: P28300
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... LOX(4015) ; mouse ... Lox(16948) ; rat ... Lox(24914)

Beschreibung

Allgemeine Beschreibung

The LOX gene is located on the human chromosome at 5q23.1.

The gene LOX (lysyl oxidase) encodes a copper-dependent amine oxidase that belongs to LOX family of proteins. These proteins contain highly conserved C- terminal mature catalytic domains that include the copper binding site, the lysyl tyrosyl quinine (LTQ) cofactor residues, and the cytokine receptor like (CRL) domain. It is present in both intercellular and intracellular locations.

Spezifität

Anti-Lysyl Oxidase (C-terminal) specifically recognizes human lysyl oxidase (LOX).

Anwendung

Anti-Lysyl Oxidase (C-terminal) antibody produced in rabbit has been used in:

• western blotting
• immunofluorescence staining
• immunohistochemistry

Biochem./physiol. Wirkung

Altered LOX gene expression and activity are linked to skin aging and senescence. Decreased LOX gene expression and activity have been associated with severe connective tissue disorders such as Ehler-Danlos syndrome, cutis laxa, and Menkes′ syndrome. Increased LOX gene expression are associated with the development of fibrotic diseases that involve connective tissue remodeling, such as atherosclerosis, scleroderma and liver cirrhosis. LOX protein also plays an important role in breast cancer metastasis.

LOX is implicated in various intracellular functions such as the regulation of cellular differentiation, cell migration and gene transcription.

The gene LOX (lysyl oxidase) encodes an extracellular matrix remodeling enzyme that catalyzes the oxidation of primary amino group of peptidyl lysine to reactive peptidyl aldehydes. It is mainly involved in the oxidation of lysine residues in elastin and collagen, resulting in the formation of covalent cross-linkages, which stabilize these fibrous proteins. It also plays a role in several cellular processes such as developmental regulation, tumor suppression, cell motility, and cellular senescence. It is involved in hypoxia-induced metastasis and serves as a therapeutic target for the treatment of metastases.

Physikalische Form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Lagerung und Haltbarkeit

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)