I1146

R³ IGF-1 human

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(e): Human IGF-1, IGF-1 Protein, Recombinant IGF-1
• UNSPSC-Code: 51111800
• NACRES: NA.77

Eigenschaften

• Biologische Quelle: human
• Qualitätsniveau: 200
• Rekombinant: expressed in E. coli
• Assay: ≥85% (HPLC)
• Form: lyophilized powder
• Wirksamkeit: ≤10 ng/mL ED₅₀
• Qualität: endotoxin tested
• Mol-Gew.: 7,676 Da
• Verpackung: pkg of 0.1 mg
• Methode(n): cell culture | mammalian: suitable
• UniProt-Hinterlegungsnummer: P05019
• Lagertemp.: 2-8°C
• Angaben zum Gen: human ... IGF1(3479)

Beschreibung

Anwendung

R3 IGF-I is a recombinant analog of insulin-like growth factor containing the complete human IGF-I amino acid sequence with substitution of Arg for Glu3. It was developed as an inexpensive, high quality potent analog of IGF for use as a growth factor supplement for serum-free or low serum cell culture.

Recombinantly produced in E. coli using a patented expression system, the peptide is harvested from cells in inclusion bodies, which are dissolved and desalted. The active molecule is properly folded under oxidizing conditions and then purified by several liquid chromatography steps.

Biochem./physiol. Wirkung

Proliferation of chinese hamster ovary CHO cells.

Sonstige Hinweise

Recombinant analog of insulin-like growth factor-I with the substitution of Arg for Glu at position 3.

Angaben zur Herstellung

Prepared from 0.1 M acetic acid

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)