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H9037

Sigma-Aldrich

Monoklonaler ANTI-HA-Antikörper

~1 mg/mL, clone HA-7, purified from hybridoma cell culture

Synonym(e):

Anti-HA, Anti-Influenza-Hämagglutinin

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About This Item

MDL-Nummer:
MFCD00803873
UNSPSC-Code:
12352203
NACRES:
NA.56

Biologische Quelle

mouse

Konjugat

TRITC conjugate

Antikörperform

purified from hybridoma cell culture

Antikörper-Produkttyp

primary antibodies

Klon

HA-7, monoclonal

Form

buffered aqueous solution

Lagerbedingungen

protect from light

Konzentration

~1 mg/mL

Methode(n)

direct immunofluorescence: 10-15 mg/mL using mammalian cells expressing HA-tagged fusion proteins

Isotyp

IgG1

Versandbedingung

dry ice

Lagertemp.

−20°C

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Allgemeine Beschreibung

Monoclonal Anti-HA is derived from the HA-7 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Monoclonal anti-HA recognizes the HA tag sequence on HA tagged fusion proteins by immunocytochemistry. The antibody reacts specifically with N- and C-terminal HA tagged fusion proteins.

Spezifität

Der Antikörper erkennt native sowie durch Denaturierung reduzierte Formen des HA-getaggten Proteins und reagiert mit am N- oder C-Endpunkt HA-getaggten Fusionsproteinen, die in E. coli oder in Säugetierzellen exprimiert werden.

Immunogen

An KLH konjugiertes synthetisches Peptid, das einem Fragment des Hämagglutinins (HA) des menschlichen Grippevirus, genannt HA-Tag, entspricht.

Anwendung

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Monoclonal Anti-HA-TRITC antibody was used to test if DOT/ICM effector SDHA is necessary for virulence of Legionella pneumophila in Galleria mellonella and A/J mice.

Physikalische Form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin 15 mM sodium azide as a preservative.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Clare R Harding et al.
Infection and immunity, 81(7), 2598-2605 (2013-05-08)
Legionella pneumophila is an intracellular bacterium that resides within amoebae and macrophages in a specialized compartment termed the Legionella-containing vacuole (LCV). As well as providing an intracellular niche for replication, the LCV helps to prevent the release of bacterial components
D J Scott et al.
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To assess the effectiveness of the surgical correction of vesico-ureteric reflux, current renal function was determined in 56 children with scarred kidneys who had undergone ureteric reimplantation between 1978 and 1983. The children were aged between 2 and 15 years
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Current opinion in biotechnology, 4(5), 520-525 (1993-10-01)
Recent advances in protein expression in E. coli have focused primarily on the enhancement of protein quality. Problems in mRNA translation such as inefficient initiation, mistranslation, frame-shifting and frame-hopping can often be addressed by altering heterologous gene-coding sequences. Fusion technology
Clare R Harding et al.
Infection and immunity, 81(11), 4261-4270 (2013-09-05)
The Dot/Icm type IV secretion system (T4SS) of Legionella pneumophila is crucial for the pathogen to survive in protozoa and cause human disease. Although more than 275 effector proteins are delivered into the host cell by the T4SS, the function
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Infection and immunity, 83(10), 3989-4002 (2015-07-29)
Legionella pneumophila is a bacterial pathogen that thrives in alveolar macrophages, causing a severe pneumonia. The virulence of L. pneumophila depends on its Dot/Icm type IV secretion system (T4SS), which delivers more than 300 effector proteins into the host, where
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