GF021

Stem Cell Factor Protein, Recombinant human

The Stem Cell Factor (SCF) Protein, Recombinant human, is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis.

• Synonym(e): SCF, Mast Cell Growth Factor, MGF, c-kit Ligand
• UNSPSC-Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.77

Eigenschaften

• Biologische Quelle: human
• Qualitätsniveau: 100
• Rekombinant: expressed in E. coli
• Assay: >98% (SDS-PAGE and HPLC)
• Spezifische Aktivität: ≥5 x 10e5 U/mg
• Hersteller/Markenname: Chemicon^®
• Verunreinigungen: <0.1 ng/μg Endotoxin (of SCF; 1EU/μg)
• Aufnahme: sample type hematopoietic stem cell(s)
• NCBI-Hinterlegungsnummer: NM_000899.3; NM_003994.4
• UniProt-Hinterlegungsnummer: P21583
• Versandbedingung: dry ice
• Angaben zum Gen: human ... KITLG(4254)

Beschreibung

Allgemeine Beschreibung

Product Source: E.coli

Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. Recombinant human SCF is an 18.4 kDa protein containing 165 amino acid residues corresponding to the soluble secreted form of SCF.

Anwendung

For most in vitro applications, SCF exerts its biological activity in the concentration range of 0.2 to 20.0 ng/mL. Responding cells are (partial list): Stem cells, BM precursor cells.

Physikalische Form

The sterile filtered solution was lyophilized from 10mM Acetic acid.

Lagerung und Haltbarkeit

The lyophilized powder, though stable for a few weeks at room temperature, is best stored at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can be further diluted into other aqueous buffers and stored at 2-8°C for 1 week or -20°C for future use. Reconstituted SCF should be stored in undiluted aliquots at -20°C. Avoid repeated freeze/thaw cycles.

Hinweis zur Analyse

Specific Activity: Chemicon′s human SCF is fully biologically active when compared to standards.The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 2 ng/mL

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1