GE17-5143-01
HiTrap® Benzamidine Fast Flow
Cytiva 17-5143-01, pack of 5 × 1 mL
About This Item
Empfohlene Produkte
ligand
p-aminobenzamidine
Verpackung
pack of 5 × 1 mL
Hersteller/Markenname
Cytiva 17-5143-01
Lagerbedingungen
(0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol)
Parameter
42 psi
Bettgröße
7 mm × 25 mm
Bettvolumen
1 mL
Säule ID
7 mm
Matrix
highly cross-linked 4% agarose
Durchschnittlicher Durchmesser
90 μm
cleaning
1-9
working range
2-8
Lagertemp.
2-8°C
Allgemeine Beschreibung
Anwendung
The medium, Benzamidine Sepharose™ 4 Fast FLow (high sub), is also available as lab packages and is an excellent choice for scale-up. The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign or FPLC™ System.
Leistungsmerkmale und Vorteile
- Effective removal of thrombin and factor Xa after tag cleavage of recombinant Proteins.
- Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates.
- HiTrap™ Benzamidine FF for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.
- High binding capacity.
- The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTA™ design or FPLC™ System.
Lagerung und Haltbarkeit
Hinweis zur Analyse
Rechtliche Hinweise
Ähnliches Produkt
Signalwort
Warning
H-Sätze
Lagerklassenschlüssel
3 - Flammable liquids
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Artikel
This page shows troubleshooting strategies for cleavage methods using GE Healthcare products.
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from GE Healthcare.
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from GE Healthcare.
This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from GE Healthcare.
Protokolle
This page shows how to remove thrombin and Factor Xa using HiTrap® Benzamidine FF (high sub) from GE Healthcare.
How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from GE Healthcare.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.
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