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Merck

G5154

Sigma-Aldrich

Glasgow Minimum Essential Medium

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(e):

GMEM

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352207
NACRES:
NA.75

product name

Glasgow Minimum Essential Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sterilität

sterile-filtered

Form

liquid

Methode(n)

cell culture | mammalian: suitable

Verunreinigungen

endotoxin, tested

Komponenten

L-glutamine: no
phenol red: 0.0213 g/L
NaHCO3: 2.75 g/L
glucose: 4.5 g/L (Dextro)

Versandbedingung

ambient

Lagertemp.

2-8°C

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Allgemeine Beschreibung

For use with adherent kidney cell lines such as baby hamster kidney cells (BHK).
Glasgow Minimum Essential Medium has been developed by modifying the Eagle′s BME medium. This medium can be used to study the genetic factors affecting cell competence.

Anwendung

Glasgow Minimum Essential Medium has been used to culture:
  • Hoxb7- green fluorescence protein (GFP) mouse embryonic stem (ES) cell line for the induction of ureteric bud differentiation
  • murine E14 Tg2a embryonic stem (mES) cells
  • transduced Chinese hamster ovary (CHO) cells for the expression and production of Siglec-1-Fc fusion proteins

Rekonstituierung

Supplement with 0.292 g/L L-glutamine and Tryptose phosphate broth solution (T 8159) at 100 ml/L of medium.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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