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Merck

F2043

Sigma-Aldrich

Monoklonaler Anti-β-Tubulin-Antikörper

clone TUB 2.1, purified from hybridoma cell culture

Synonym(e):

Monoclonal Anti-β-Tubulin

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Konjugat

FITC conjugate

Antikörperform

purified from hybridoma cell culture

Antikörper-Produkttyp

primary antibodies

Klon

TUB 2.1, monoclonal

Form

buffered aqueous solution

Mol-Gew.

antigen 55 kDa

Speziesreaktivität

human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster

Lagerbedingungen

protect from light

Methode(n)

direct immunofluorescence: 1:25 using cultured BHK cells

Isotyp

IgG1

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

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Allgemeine Beschreibung

β-Tubulin belongs to the subfamily of tubulins. It has two β-sheets enclosed by α-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site and a C-terminal domain, which has the binding surface for molecular motor proteins. Human β-tubulin consists of seven isoforms (βI (class I), βII (class II), βIII (class III), βIVa (class IVa), βIVb (class IVb), βV (class V), and βVI (class VI)). TUBB1 (tubulin beta class I) is located on human chromosome 6p21.33.(10)
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton. Tubulin is a heterodimer, which consists of α-tubulin and β-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology. The most studied tubulins have been isolated from vertebrate brains. The microtubules can be viewed in immunofluorescent microscopy, which enables the observation of the intracellular organization of proteins that are in the form of a supramolecular structure.

Spezifität

Der Antikörper erkennt alle fünf Isoformen von β-Tubulin (β1-β5). Er reagiert beim Immunblotting mit den β-Lc- und β-Sc-Fragmenten am Carboxy-Terminus des β−Tubulins. Der Antikörper kann zur Lokalisierung von β−-Tubulin in kultivierten Zellen oder Gewebeschnitten verwendet werden.

Immunogen

Tubulin aus Rattenhirn.

Anwendung

FITC-β tubulin antibody may be used for detection ajnd localization of β-tubulin by direct labeling in a variety of eukaryotic cells ranging from human embryonic kidney cells (HEK293T) to cells of azuki bean epicotyl. by immunohistochemical and immunocytochemical assays.
Monoclonal Anti-β-Tubulin-FITC antibody has been used:
  • in the evaluation of the status of the meiotic spindle and the arrangement of chromosomes
  • in immunofluorescence studies
  • in immunofluorescence microscopy
  • in spindle and chromosome imagery(6)

Biochem./physiol. Wirkung

Mutation in β-Tubulin gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. β-1 tubulin, also known as class VI or TUBB1, plays a vital role in platelet production and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP).

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Transient increase in the transcript levels of gamma-tubulin complex genes during reorientation of cortical microtubules by gravity in azuki bean (Vigna angularis) epicotyls
Soga K, et al.
Journal of Plant Research, 121(5), 493-498 (2008)
Abraham L Kierszenbaum
Molecular reproduction and development, 62(1), 1-3 (2002-04-05)
Two microtubule-containing structures are assembled during spermiogenesis: a transient manchette and a stable axoneme. Both structures contain microtubules enriched in posttranslationally modified tubulins. Despite the existence of a spectrum of tubulin isotypes postulated by the multi-tubulin hypothesis, further extended by
Peloruside-and laulimalide-resistant human ovarian carcinoma cells have betaI-tubulin mutations and altered expression of betaII-and betaIII-tubulin isotypes
Kanakkanthara A, et al.
Molecular Cancer Therapeutics, 393(6681), molcanther-molcan1057 (2011)
Changes to the meiotic spindle and zona pellucida of mature mouse oocytes following different cryopreservation methods
Ko C S, et al.
Animal Reproduction Science, 105(3-4), 272-282 (2008)
J C Bruusgaard et al.
Journal of applied physiology (Bethesda, Md. : 1985), 100(6), 2024-2030 (2006-02-25)
We have recently published a new technique for visualizing nuclei in living muscle fibers of intact animals, based on microinjection of labeled DNA into single myofibers, excluding satellite cells (Bruusgaard JC, Liestol K, Ekmark M, Kollstad K, and Gundersen K.

Artikel

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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